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Literature summary for 2.1.3.2 extracted from
- Aspartate carbamyltransferase (Pseudomonas fluorescens) (1978), Methods Enzymol., 51, 51-58.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Pseudomonas fluorescens |  |
| CDP | - |
Pseudomonas fluorescens | |
| CTP | - |
Pseudomonas fluorescens | |
| diphosphate | - |
Pseudomonas fluorescens | |
| phosphate | - |
Pseudomonas fluorescens | |
| ribose-5-P | - |
Pseudomonas fluorescens | |
| UTP | - |
Pseudomonas fluorescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 180000 | - |
2 * 180000, SDS-PAGE | Pseudomonas fluorescens |
| 360000 | - |
sedimentation equilibrium | Pseudomonas fluorescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoylphosphate + L-aspartate | Pseudomonas fluorescens | - |
phosphate + N-carbamoyl-L-aspartate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Streptomycin sulfate, ammonium sulfate, Sephadex G-200, hydroxylapatite, preparative gel electrophoresis | Pseudomonas fluorescens |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| dialysis after SDS-denaturation, 1% of original activity is restored | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoyl phosphate + L-aspartate | - |
Pseudomonas fluorescens | phosphate + N-carbamoyl-L-aspartate | - |
? | |
| carbamoylphosphate + L-aspartate | - |
Pseudomonas fluorescens | phosphate + N-carbamoyl-L-aspartate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 180000, SDS-PAGE | Pseudomonas fluorescens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
diphosphate | when carbamoyl phosphate is limiting | Pseudomonas fluorescens | |
| 0.007 | - |
CTP | when carbamoyl phosphate is limiting | Pseudomonas fluorescens | |
| 0.0085 | - |
ATP | when carbamoyl phosphate is limiting | Pseudomonas fluorescens | |
| 0.0085 | - |
UTP | when carbamoyl phosphate is limiting | Pseudomonas fluorescens | |
| 0.1 | - |
phosphate | when carbamoyl phosphate is limiting | Pseudomonas fluorescens | |
| 0.1 | - |
CDP | when carbamoyl phosphate is limiting | Pseudomonas fluorescens | |
| 1.5 | - |
ribose-5-P | when carbamoyl phosphate is limiting | Pseudomonas fluorescens | |
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