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Literature summary for 2.1.3.2 extracted from

  • Van Boxstael, S.; Maes, D.; Cunin, R.
    Aspartate transcarbamylase from the hyperthermophilic archaeon Pyrococcus abyssi. Insights into cooperative and allosteric mechanisms (2005), Febs J., 272, 2670-2683.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ATP allosteric activator, up to 35% increase in Vmax Pyrococcus abyssi
additional information kinetic analysis of properties of allosteric effectors alone and in combination with each other Pyrococcus abyssi

Inhibitors

Inhibitors Comment Organism Structure
CTP allosteric inhibitor Pyrococcus abyssi
additional information kinetic analysis of properties of allosteric effectors alone and in combination with each other Pyrococcus abyssi
UTP allosteric inhibitor Pyrococcus abyssi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
Carbamoyl phosphate 37°C Pyrococcus abyssi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
6 * 35000 + 6 * 17000, 2C3/3R2 holoenzyme, calculated Pyrococcus abyssi
35000
-
6 * 35000 + 6 * 17000, 2C3/3R2 holoenzyme, calculated Pyrococcus abyssi
301000
-
gel filtration Pyrococcus abyssi

Organism

Organism UniProt Comment Textmining
Pyrococcus abyssi
-
recombinant protein
-

Reaction

Reaction Comment Organism Reaction ID
carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate cooperative mechanism of substrate binding Pyrococcus abyssi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-aspartate
-
Pyrococcus abyssi phosphate + N-carbamoyl-L-aspartate
-
?

Subunits

Subunits Comment Organism
dodecamer 6 * 35000 + 6 * 17000, 2C3/3R2 holoenzyme, calculated Pyrococcus abyssi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
half-life 260 min Pyrococcus abyssi
94
-
half-life 190 min Pyrococcus abyssi
98
-
half-life 100 min Pyrococcus abyssi