Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ornithine | competitive vs. carbamoylphosphate in the presence of phosphate | Spinacia oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
3 * 40000, SDS-PAGE | Spinacia oleracea |
118000 | - |
gel filtration | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, heat, activated charcoal, delta-N-(phosphonacetyl)-L-ornithine affinity chromatography | Spinacia oleracea |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
carbamoyl phosphate + L-ornithine = phosphate + L-citrulline | ordered bi-bi-sequential reaction mechanism, carbamoylphosphate binds first followed by L-ornithine, L-citrulline leaves first followed by phosphate | Spinacia oleracea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
436 | - |
- |
Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-ornithine | - |
Spinacia oleracea | phosphate + L-citrulline | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 40000, SDS-PAGE | Spinacia oleracea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
56 | - |
half-live: 99 min | Spinacia oleracea |
58 | - |
half-life: 60 min | Spinacia oleracea |
64 | - |
half-life: 11 min | Spinacia oleracea |
68 | - |
half-life: 10.5 min, half-life increases to 45 min in the presence of 50 mM ornithine | Spinacia oleracea |