Literature summary for 2.1.3.3 extracted from

El Alami, M.; Dubois, E.; Oudjama, Y.; Tricot, C.; Wouters, J.; Stalon, V.; Messenguy, F.
Yeast epiarginase regulation, an enzyme-enzyme activity control: identification of residues of L-ornithine carbamoyltransferase and arginase responsible for enzyme catalytic and regulatory activities (2003), J. Biol. Chem., 278, 21550-21558.

Protein Variants

Protein Variants	Comment	Organism
D182N	greatly reduced affinity for L-ornithine, no interaction with arginase	Saccharomyces cerevisiae
E123A	little change in activity, or in sensitivity to arginase	Saccharomyces cerevisiae
E123S	little change in activity, or in sensitivity to arginase	Saccharomyces cerevisiae
E256A	greatly reduced affinity for L-ornithine, impaired interaction with arginase	Saccharomyces cerevisiae
E256Q	greatly reduced affinity for L-ornithine, impaired interaction with arginase	Saccharomyces cerevisiae
G181R	no residual activity	Saccharomyces cerevisiae
K260A	important in mediating sensitivity to L-ornithine and arginase	Saccharomyces cerevisiae
K260R	important in mediating sensitivity to L-ornithine and arginase	Saccharomyces cerevisiae
K263R	important in mediating sensitivity to L-ornithine and arginase	Saccharomyces cerevisiae
K265A	important in mediating sensitivity to L-ornithine and arginase	Saccharomyces cerevisiae
K265R	important in mediating sensitivity to L-ornithine and arginase	Saccharomyces cerevisiae
K268A	important in mediating sensitivity to L-ornithine and arginase	Saccharomyces cerevisiae
K268R	important in mediating sensitivity to L-ornithine and arginase	Saccharomyces cerevisiae
K289S	greatly reduced affinity for L-ornithine, impaired interaction with arginase	Saccharomyces cerevisiae
L290Q	reduced activity, little change in sensitivity to arginase	Saccharomyces cerevisiae
L290S	reduced activity, little change in sensitivity to arginase	Saccharomyces cerevisiae
N184Q	greatly reduced affinity for L-ornithine, impaired interaction with arginase	Saccharomyces cerevisiae
N185Q	greatly reduced affinity for L-ornithine, impaired interaction with arginase	Saccharomyces cerevisiae
Q294P	little change in activity, or in sensitivity to arginase	Saccharomyces cerevisiae
T68G	greatly reduced activity, no change in sensitivity to arginase	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
arginase	500 units, 14% remaining activity	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.05	-	L-ornithine	mutant K268A, pH 8.0, 30°C	Saccharomyces cerevisiae
1	-	L-ornithine	mutant K260A, pH 8.0, 30°C	Saccharomyces cerevisiae
1.1	-	L-ornithine	mutant K265R, pH 8.0, 30°C	Saccharomyces cerevisiae
1.1	-	L-ornithine	mutant K268R, pH 8.0, 30°C	Saccharomyces cerevisiae
1.2	-	L-ornithine	mutant E256Q, pH 8.0, 30°C	Saccharomyces cerevisiae
1.2	-	L-ornithine	mutant K260R, pH 8.0, 30°C	Saccharomyces cerevisiae
1.2	-	L-ornithine	mutant K265A, pH 8.0, 30°C	Saccharomyces cerevisiae
1.3	-	L-ornithine	mutant Q294P, pH 8.0, 30°C	Saccharomyces cerevisiae
1.6	-	L-ornithine	wild-type, pH 8.0, 30°C	Saccharomyces cerevisiae
2	-	L-ornithine	mutant L290Q, pH 8.0, 30°C	Saccharomyces cerevisiae
2	-	L-ornithine	mutant L290S, pH 8.0, 30°C	Saccharomyces cerevisiae
2.1	-	L-ornithine	mutant E123S, pH 8.0, 30°C	Saccharomyces cerevisiae
2.6	-	L-ornithine	mutant E123A, pH 8.0, 30°C	Saccharomyces cerevisiae
2.8	-	L-ornithine	mutant C191M/C194N/F197V, pH 8.0, 30°C	Saccharomyces cerevisiae
3.4	-	L-ornithine	mutant T68G, pH 8.0, 30°C	Saccharomyces cerevisiae
10	-	L-ornithine	mutant E256A, pH 8.0, 30°C	Saccharomyces cerevisiae
10	-	L-ornithine	mutant K289S, pH 8.0, 30°C	Saccharomyces cerevisiae
20	-	L-ornithine	mutant K263R, pH 8.0, 30°C	Saccharomyces cerevisiae
30	-	L-ornithine	mutant K263A, pH 8.0, 30°C	Saccharomyces cerevisiae
34	-	L-ornithine	mutant N184Q, pH 8.0, 30°C	Saccharomyces cerevisiae
49	-	L-ornithine	mutant D182N, pH 8.0, 30°C	Saccharomyces cerevisiae
350	-	L-ornithine	mutant N185Q, pH 8.0, 30°C	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.28	-	mutant T68G, pH 8.0, 30°C	Saccharomyces cerevisiae
2.83	-	mutant K289S, pH 8.0, 30°C	Saccharomyces cerevisiae
4.16	-	mutant C191M/C194N/F197V, pH 8.0, 30°C	Saccharomyces cerevisiae
4.66	-	mutant L290S, pH 8.0, 30°C	Saccharomyces cerevisiae
7.5	-	mutant K263A, pH 8.0, 30°C	Saccharomyces cerevisiae
12.33	-	mutant N184Q, pH 8.0, 30°C	Saccharomyces cerevisiae
12.66	-	mutant E256Q, pH 8.0, 30°C	Saccharomyces cerevisiae
13.3	-	mutant L290Q, pH 8.0, 30°C	Saccharomyces cerevisiae
14.83	-	mutant K263R, pH 8.0, 30°C	Saccharomyces cerevisiae
15	-	mutant E256A, pH 8.0, 30°C	Saccharomyces cerevisiae
16.16	-	mutant D182N, pH 8.0, 30°C	Saccharomyces cerevisiae
16.83	-	mutant K265A, pH 8.0, 30°C	Saccharomyces cerevisiae
23.38	-	mutant E123S, pH 8.0, 30°C	Saccharomyces cerevisiae
23.63	-	mutant K265R, pH 8.0, 30°C	Saccharomyces cerevisiae
25	-	mutant K268A, pH 8.0, 30°C	Saccharomyces cerevisiae
25	-	mutant N185Q, pH 8.0, 30°C	Saccharomyces cerevisiae
25.16	-	mutant K260R, pH 8.0, 30°C	Saccharomyces cerevisiae
25.3	-	mutant E123A, pH 8.0, 30°C	Saccharomyces cerevisiae
25.73	-	mutant K268R, pH 8.0, 30°C	Saccharomyces cerevisiae
25.83	-	mutant K260A, pH 8.0, 30°C	Saccharomyces cerevisiae
26.12	-	wild-type, pH 8.0, 30°C	Saccharomyces cerevisiae
48.66	-	mutant Q294P, pH 8.0, 30°C	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
carbamoyl phosphate + L-ornithine	-	Saccharomyces cerevisiae	phosphate + L-citrulline	-	?

Subunits

Subunits	Comment	Organism
More	in presence of ornitthine and arginine, enzyme forms a complex with arginase of 200 kDa	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
otcase	-	Saccharomyces cerevisiae