Literature summary for 2.1.3.3 extracted from

Baur, H.; Tricot, C.; Stalon, V.; Haas, D.
Converting catabolic ornithine carbamoyltransferase to an anabolic enzyme (1990), J. Biol. Chem., 265, 14728-14731.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Escherichia coli
expression in Pseudomonas aeruginosa	Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
E106A	mutant arcB6240, with strongly reduced cooperativity for carbamoyl phosphate and anabolic activity	Pseudomonas aeruginosa
E106G	mutant arcB6254, with strongly reduced cooperativity for carbamoyl phosphate and anabolic activity	Pseudomonas aeruginosa
Q106E	mutant to study the carbamoyl phosphate cooperativity on the anabolic OTCase	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.06	-	Carbamoyl phosphate	Q106E	Escherichia coli
0.36	-	Carbamoyl phosphate	wild type	Escherichia coli
0.8	-	ornithine	wild type, pH 7.25	Pseudomonas aeruginosa
0.85	-	ornithine	-	Escherichia coli
1.8	-	ornithine	mutant arcB6254, pH 7.25	Pseudomonas aeruginosa
1.85	-	ornithine	mutant arcB6240, pH 7.25	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Pseudomonas aeruginosa	-	-	-
Pseudomonas aeruginosa PAO532	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
carbamoyl phosphate + L-ornithine	-	Escherichia coli	phosphate + citrulline	-	?
carbamoyl phosphate + L-ornithine	-	Pseudomonas aeruginosa	phosphate + citrulline	-	?
carbamoyl phosphate + L-ornithine	-	Pseudomonas aeruginosa PAO532	phosphate + citrulline	-	?

Synonyms

Synonyms	Comment	Organism
otcase	-	Escherichia coli
otcase	-	Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.25	-	wild type	Pseudomonas aeruginosa
8.2	-	mutant arcB6240	Pseudomonas aeruginosa
8.5	-	mutant arcB6254	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)