Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Enterococcus faecalis |
gene ef0732, gene cluster organization, DNA and amino acid sequence determination and analysis, overexpression of His-tagged enzyme in Escherichia coli | Enterococcus faecalis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in presence of inhibitor N-(phosphonoacetyl)-putrescine, X-ray diffraction structure determination and analysis at 3.0 A resolution | Enterococcus faecalis |
using the sparse-matrix sampling vapor-diffusion method. The addition of N-(phosphonoacetyl)-putrescine to the crystallization drop strongly improves the results of the crystallization | Enterococcus faecalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-(phosphonoacetyl)-putrescine | i.e. PAPU, highly potent and selective inhibitor | Enterococcus faecalis | |
phosphonoacetyl-L-aspartate | - |
Enterococcus faecalis | |
phosphonoacetyl-L-ornithine | - |
Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
putrescine | - |
Enterococcus faecalis | |
0.058 | - |
Carbamoyl phosphate | - |
Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration | Enterococcus faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-ornithine | Enterococcus faecalis | low activity, reaction of EC 2.1.3.3 | phosphate + N-carbamoyl-L-ornithine | - |
? | |
carbamoyl phosphate + L-ornithine | Enterococcus faecalis ATCC 700802 | low activity, reaction of EC 2.1.3.3 | phosphate + N-carbamoyl-L-ornithine | - |
? | |
carbamoyl phosphate + putrescine | Enterococcus faecalis | - |
phosphate + N-carbamoyl-putrescine | - |
? | |
carbamoyl phosphate + putrescine | Enterococcus faecalis ATCC 700802 | - |
phosphate + N-carbamoyl-putrescine | - |
? | |
additional information | Enterococcus faecalis | the enzyme catalyzes the second of three steps of ATP synthesis from agmatine | ? | - |
? | |
additional information | Enterococcus faecalis ATCC 700802 | the enzyme catalyzes the second of three steps of ATP synthesis from agmatine | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Enterococcus faecalis | - |
strain SD10, gene ef0732 | - |
Enterococcus faecalis ATCC 700802 | - |
strain SD10, gene ef0732 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Enterococcus faecalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
460 | - |
non-recombinant enzyme from Enterococcus faecalis | Enterococcus faecalis |
597 | - |
recombinant enzyme from Enterococcus faecalis | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + cadaverine | only weak activity with cadaverine a substrate | Enterococcus faecalis | phosphate + N-carbamoylcadaverine | - |
? | |
carbamoyl phosphate + cadaverine | only weak activity with cadaverine a substrate | Enterococcus faecalis ATCC 700802 | phosphate + N-carbamoylcadaverine | - |
? | |
carbamoyl phosphate + L-ornithine | low activity, reaction of EC 2.1.3.3 | Enterococcus faecalis | phosphate + N-carbamoyl-L-ornithine | - |
? | |
carbamoyl phosphate + L-ornithine | low activity, reaction of EC 2.1.3.3 | Enterococcus faecalis ATCC 700802 | phosphate + N-carbamoyl-L-ornithine | - |
? | |
carbamoyl phosphate + ornithine | only weak activity with ornithine as a substrate | Enterococcus faecalis | phosphate + N-carbamoylornithine | - |
? | |
carbamoyl phosphate + putrescine | - |
Enterococcus faecalis | phosphate + N-carbamoylputrescine | - |
? | |
carbamoyl phosphate + putrescine | - |
Enterococcus faecalis ATCC 700802 | phosphate + N-carbamoylputrescine | - |
? | |
carbamoyl phosphate + putrescine | - |
Enterococcus faecalis | phosphate + N-carbamoyl-putrescine | - |
? | |
carbamoyl phosphate + putrescine | - |
Enterococcus faecalis ATCC 700802 | phosphate + N-carbamoyl-putrescine | - |
? | |
additional information | the enzyme catalyzes the second of three steps of ATP synthesis from agmatine | Enterococcus faecalis | ? | - |
? | |
additional information | the enzyme catalyzes the second of three steps of ATP synthesis from agmatine | Enterococcus faecalis ATCC 700802 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | - |
Enterococcus faecalis |
trimer | crystal strucutre | Enterococcus faecalis |
Synonyms | Comment | Organism |
---|---|---|
PTC | - |
Enterococcus faecalis |
putrescine transcarbamylase | - |
Enterococcus faecalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Enterococcus faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Enterococcus faecalis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00001 | - |
N-(phosphonoacetyl)-putrescine | recombinant enzyme | Enterococcus faecalis | |
10 | - |
N-(phosphonoacetyl)-putrescine | - |
Enterococcus faecalis |