Literature summary for 2.2.1.1 extracted from

Matosevic, S.; Lye, G.J.; Baganz, F.
Design and characterization of a prototype enzyme microreactor: Quantification of immobilized transketolase kinetics (2009), Biotechnol. Prog., 26, 118-126.

Search for more literature for 2.2.1.1

Application

Application	Comment	Organism
synthesis	desing of an enzyme microreaktor by reversible immobilization of His6-tagged enzyme a 200-microm ID fused silica capillary for quantitative kinetic analysis. Transketolase kinetic parameters in the mircoreactor are comparable with those measured in free solution. Quantitative elution of the immobilized transketolase and the regeneration and reuse of the derivatized capillary over five cycles are possible	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli, His-taggged protein	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
26	-	lithium beta-hydroxypyruvate	pH 7.0, 25°C	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
lithium beta-hydroxypyruvate + glycolaldehyde	-	Escherichia coli	L-erythrulose + ?	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
410000	-	lithium beta-hydroxypyruvate	pH 7.0, 25°C	Escherichia coli

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Release 2023.1 (January 2023)