Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
D-xylulose 5-phosphate | pH 7.5, 22°C | Trypanosoma brucei | |
0.8 | - |
D-ribose 5-phosphate | pH 7.5, 22°C | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | main localization | Trypanosoma brucei | 5829 | - |
glycosome | to lesser extent than in cytosol | Trypanosoma brucei | 20015 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Trypanosoma brucei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | transketolase activity is absent from the long slender bloodstream form of the parasite and the protein is not detectable in this life cycle stage, with the RNA present only at low abundance | Trypanosoma brucei | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylulose 5-phosphate + D-ribose 5-phosphate | - |
Trypanosoma brucei | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
? |
General Information | Comment | Organism |
---|---|---|
physiological function | gene knock out from procyclic Trypanosoma brucei results in loss of transketolase activity ,but no growth phenotype is evident in the null mutants. Metabolite profiling to compare wild type and TKT null mutants reveals substantial increases in transketolase substrate metabolites coupled to loss of sedoheptulose 7-phosphate | Trypanosoma brucei |