Literature summary for 2.2.1.1 extracted from

Lukacik, P.; Lobley, C.M.; Bumann, M.; Arena de Souza, V.; Owens, R.J.; OToole, P.W.; Walsh, M.A.
High-resolution structures of Lactobacillus salivarius transketolase in the presence and absence of thiamine pyrophosphate (2015), Acta Crystallogr. Sect. F, 71, 1327-1334.

Search for more literature for 2.2.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta pLysS (DE3) cells	Ligilactobacillus salivarius

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with thiamine diphosphate, hanging drop vapor diffusion method, enzyme in complex with thiamine diphosphate using 15% (w/v) PEG 3350, 0.1 M sodium acetate, 0.1 M bis-Tris propane pH 7.5, apoprotein using 20% (w/v) PEG 3350, 0.2 M NaCl	Ligilactobacillus salivarius

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Ligilactobacillus salivarius

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70400	-	2 * 70400, multi-angle laser light scattering	Ligilactobacillus salivarius
138800	-	gel filtration	Ligilactobacillus salivarius

Organism

Organism	UniProt	Comment	Textmining
Ligilactobacillus salivarius	Q1WQU8	-	-
Ligilactobacillus salivarius UCC118	Q1WQU8	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel-chelation column chromatography and gel filtration	Ligilactobacillus salivarius

Subunits

Subunits	Comment	Organism
homodimer	2 * 70400, multi-angle laser light scattering	Ligilactobacillus salivarius

Synonyms

Synonyms	Comment	Organism
TktA	-	Ligilactobacillus salivarius

Cofactor

Cofactor	Comment	Organism	Structure
thiamine diphosphate	-	Ligilactobacillus salivarius

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Release 2023.1 (January 2023)