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Literature summary for 2.2.1.2 extracted from
- Transaldolase B of Escherichia coli K-12: cloning of its gene, talB, and characterization of the enzyme from recombinant strains (1995), J. Bacteriol., 177, 5930-5936.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-arabinose-5-phosphate | - |
Escherichia coli |  |
| L-glyceraldehyde | - |
Escherichia coli | |
| phosphate | - |
Escherichia coli | |
| Tris-HCl | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.038 | - |
glyceraldehyde 3-phosphate | - |
Escherichia coli | |
| 0.09 | - |
D-erythrose 4-phosphate | - |
Escherichia coli | |
| 0.285 | - |
sedoheptulose 7-phosphate | - |
Escherichia coli | |
| 1.2 | - |
D-fructose 6-phosphate | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
alpha2, 2 * 35000, SDS-PAGE | Escherichia coli |
| 70000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K12 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,5-D-threo-diketohexose phosphate + D-glyceraldehyde 3-phosphate | - |
Escherichia coli | ? | - |
? | |
| D-erythrose + sedoheptulose 7-phosphate | - |
Escherichia coli | ? | - |
? | |
| D-erythrose 4-phosphate + sedoheptulose 7-phosphate | - |
Escherichia coli | ? | - |
? | |
| D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate | - |
Escherichia coli | ? | - |
? | |
| D-fructose 6-phosphate + erythrose 4-phosphate | - |
Escherichia coli | sedoheptulose 7-phosphate + glyceraldehyde 3-phosphate | - |
? | |
| D-glyceraldehyde + sedoheptulose 7-phosphate | - |
Escherichia coli | ? | - |
? | |
| hydroxypyruvic aldehyde + sedoheptulose 7-phosphate | - |
Escherichia coli | ? | - |
? | |
| L-glyceraldehyde 3-phosphate + sedoheptulose 7-phosphate | - |
Escherichia coli | ? | - |
? | |
| L-sorbose 6-phosphate + D-glyceraldehyde 3-phosphate | - |
Escherichia coli | ? | - |
? | |
| sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
Escherichia coli | D-erythrose 4-phosphate + D-fructose 6-phosphate | - |
r | |
| sedoheptulose phosphate + D-glyceraldehyde 3-phosphate | - |
Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha2, 2 * 35000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 40 | - |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
above 50°C rapid loss in activity | Escherichia coli |
| 55 | - |
total inactivation of enzyme | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9.5 | - |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | - |
Escherichia coli |
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