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Literature summary for 2.2.1.4 extracted from

  • Yokota, A.; Sasajima, K.
    Enzymatic formation of a new monosaccharide, 1-deoxy-D-altro-heptulose phosphate, from DL-acetoin and D-ribose 5-phosphate by a transketolase mutant of Bacillus pumilus (1983), Agric. Biol. Chem., 47, 1545-1553.
No PubMed abstract available

General Stability

General Stability Organism
thiamine diphosphate, or Mg2+, or 2-mercaptoethanol stabilizes Bacillus pumilus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Bacillus pumilus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Bacillus pumilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
gel filtration Bacillus pumilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxybutan-2-one + D-ribose 5-phosphate Bacillus pumilus i.e. acetoin acetaldehyde + 1-deoxy-D-altro-heptulose 7-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Bacillus pumilus
-
transketolase mutant strain BG2532
-

Purification (Commentary)

Purification (Comment) Organism
partial Bacillus pumilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0145
-
-
Bacillus pumilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxybutan-2-one + D-ribose 5-phosphate i.e. acetoin Bacillus pumilus acetaldehyde + 1-deoxy-D-altro-heptulose 7-phosphate
-
?
methylacetoin + D-ribose 5-phosphate
-
Bacillus pumilus acetone + 1-deoxy-D-altro-heptulose 7-phosphate
-
?
additional information no substrates are acetaldehyde, pyruvate, acetone, hydroxyacetone, acetylacetone i.e. 2,4-pentanedione, propanetriol, diacetyl i.e. 2,3-butanedione, 2,3-butanediol, 1,2,3-butanetriol, 2-butanone Bacillus pumilus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bacillus pumilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
stable for 1 h at pH 6.0-8.0 in the presence of cofactors and 2-mercaptoethanol Bacillus pumilus
80
-
inactivation after 3 min Bacillus pumilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
Bacillus pumilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 stable for 1 h at 37°C in the presence of cofactors and 2-mercaptoethanol Bacillus pumilus

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate requirement, thiamine diphosphate protein Bacillus pumilus