General Stability | Organism |
---|---|
thiamine diphosphate, or Mg2+, or 2-mercaptoethanol stabilizes | Bacillus pumilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Bacillus pumilus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Bacillus pumilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
gel filtration | Bacillus pumilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybutan-2-one + D-ribose 5-phosphate | Bacillus pumilus | i.e. acetoin | acetaldehyde + 1-deoxy-D-altro-heptulose 7-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus pumilus | - |
transketolase mutant strain BG2532 | - |
Purification (Comment) | Organism |
---|---|
partial | Bacillus pumilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0145 | - |
- |
Bacillus pumilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybutan-2-one + D-ribose 5-phosphate | i.e. acetoin | Bacillus pumilus | acetaldehyde + 1-deoxy-D-altro-heptulose 7-phosphate | - |
? | |
methylacetoin + D-ribose 5-phosphate | - |
Bacillus pumilus | acetone + 1-deoxy-D-altro-heptulose 7-phosphate | - |
? | |
additional information | no substrates are acetaldehyde, pyruvate, acetone, hydroxyacetone, acetylacetone i.e. 2,4-pentanedione, propanetriol, diacetyl i.e. 2,3-butanedione, 2,3-butanediol, 1,2,3-butanetriol, 2-butanone | Bacillus pumilus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus pumilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
stable for 1 h at pH 6.0-8.0 in the presence of cofactors and 2-mercaptoethanol | Bacillus pumilus |
80 | - |
inactivation after 3 min | Bacillus pumilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Bacillus pumilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | stable for 1 h at 37°C in the presence of cofactors and 2-mercaptoethanol | Bacillus pumilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | requirement, thiamine diphosphate protein | Bacillus pumilus |