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Literature summary for 2.2.1.6 extracted from

  • Chang, A.K.; Duggleby, R.G.
    Herbicide-resistant forms of Arabidopsis thaliana acetohydroxyacid synthase: characterization of the catalytic properties and sensitivity to inhibitors of four defined mutants (1998), Biochem. J., 333, 765-777.
No PubMed abstract available
Search for more literature for 2.2.1.6

Protein Variants

Protein Variants Comment Organism
A122V reduced affinity for all Mg2+, thiamine diphosphate and FAD Arabidopsis thaliana
S653N binds FAD more strongly than the wild-type enzyme Arabidopsis thaliana
W574L insensitive to sulfonurea herbicides Arabidopsis thaliana
W574S reduction in sensitivity to sulfonurea herbicides compared to the wild-type enzyme Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate Arabidopsis thaliana first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 mutant enzymes A122V, W574S, W574L and S653N
-

Purification (Commentary)

Purification (Comment) Organism
mutant enzymes A122V, W574S, W574L and S653N expressed in Escherichia coli Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4
-
 mutant enzyme W574L Arabidopsis thaliana
8.51
-
 mutant S653N Arabidopsis thaliana
11
-
 mutant W574 Arabidopsis thaliana
12
-
 mutant enzyme A122V Arabidopsis thaliana

Storage Stability

Storage Stability Organism
-70°C, 21 days, 15% loss of activity of mutant enzyme A122V, less than 1% loss of activity of mutant enzymes W574S and S653N, 3% loss of activity of mutant enzyme W574L Arabidopsis thaliana
4°C, 21 days, 30% loss of activity of mutant enzyme A122V, 6% loss of activity of mutant enzyme W574S, 20% loss of activity of mutant enzyme W574L, 18% loss of activity of mutant enzyme S653N Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
 Arabidopsis thaliana 2-acetolactate + CO2
-
 ?
pyruvate first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile Arabidopsis thaliana ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 enzyme from mutant A122V, W574S, W574L and S653N Arabidopsis thaliana