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Literature summary for 2.2.1.6 extracted from
- Isolation, characterization, and physiological role of the pyruvate dehydrogenase complex and alpha-acetolactate synthase of Lactococcus lactis subsp. lactis bv. diacetylactis (1992), J. Bacteriol., 174, 4838.-4841.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Lactococcus lactis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 50 | - |
pyruvate | - |
Lactococcus lactis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
x * 62000, SDS-PAGE | Lactococcus lactis |
| 172000 | - |
gel filtration | Lactococcus lactis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate | Lactococcus lactis | constitutive high expression, the enzyme is active only under conditions of pyruvate excess | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactococcus lactis | - |
subsp. lactis bv, diacetylactis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Lactococcus lactis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20% glycerol, stable for 2 months | Lactococcus lactis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate | - |
Lactococcus lactis | 2-acetolactate + CO2 | - |
? | |
| pyruvate | constitutive high expression, the enzyme is active only under conditions of pyruvate excess | Lactococcus lactis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000, SDS-PAGE | Lactococcus lactis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Lactococcus lactis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | required as cofactor | Lactococcus lactis | |
| thiamine diphosphate | Km: 0.0032 mM | Lactococcus lactis | |
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