Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
isozymes AHAS I, AHAS II, AHAS III | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.06 | - |
catalytic subunit ilvI, regulatory subunit ilvN, 37°C, pH 7.6 | Escherichia coli |
1.3 | - |
catalytic subunit ilvB, regulatory subunit ilvB, 37°C, pH 7.6 | Escherichia coli |
9.3 | - |
catalytic subunit ilvI, regulatory subunit ilvM, 37°C, pH 7.6 | Escherichia coli |
9.4 | - |
wild-type AHAS III, pH 7.6, 37°C | Escherichia coli |
20.9 | - |
wild-type AHAS III, truncated regulatory subunit ilvH-DELTA 86, pH 7.6, 37°C | Escherichia coli |
32 | - |
catalytic subunit ilvB, truncated regulatory subunit ilvB-DELTA86, 37°C, pH 7.6 | Escherichia coli |
49.8 | - |
catalytic subunit ilvB, regulatory subunit ilvM, 37°C, pH 7.6 | Escherichia coli |
50.8 | - |
wild-type AHAS I, 37°C, pH 7.6 | Escherichia coli |
60.4 | - |
wild-type AHAS II, 37°C, pH 7.6 | Escherichia coli |
Subunits | Comment | Organism |
---|---|---|
More | isozyme AHAS I, catalytic subunit ilvB, regulatory subunit ilvN. AHAS II, catalytic subunit ilvG, regulatory subunit ilvM. Isozyme AHAS III, catalytic subunit ilvI, regulatory subunit ilvH. AHAS II regulatory subunit ilvM is able to activate the catalytic subunits of all three of the isozymes, and the truncated AHAS III regulatory subunits ilvH-DELTA80, ilvH-DELTA86 and ilvH-DELTA89 are able to activate the catalytic subunits of both AHAS I and AHAS III. Contrary to wild-type, none of the heterologously activated enzymes have any feedback sensitivity | Escherichia coli |