Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.2.1.6 extracted from

  • Vyazmensky, M.; Zherdev, Y.; Slutzker, A.; Belenky, I.; Kryukov, O.; Barak, Z.; Chipman, D.M.
    Interactions between large and small subunits of different acetohydroxyacid synthase isozymes of Escherichia coli (2009), Biochemistry, 48, 8731-8737.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
isozymes AHAS I, AHAS II, AHAS III
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06
-
catalytic subunit ilvI, regulatory subunit ilvN, 37°C, pH 7.6 Escherichia coli
1.3
-
catalytic subunit ilvB, regulatory subunit ilvB, 37°C, pH 7.6 Escherichia coli
9.3
-
catalytic subunit ilvI, regulatory subunit ilvM, 37°C, pH 7.6 Escherichia coli
9.4
-
wild-type AHAS III, pH 7.6, 37°C Escherichia coli
20.9
-
wild-type AHAS III, truncated regulatory subunit ilvH-DELTA 86, pH 7.6, 37°C Escherichia coli
32
-
catalytic subunit ilvB, truncated regulatory subunit ilvB-DELTA86, 37°C, pH 7.6 Escherichia coli
49.8
-
catalytic subunit ilvB, regulatory subunit ilvM, 37°C, pH 7.6 Escherichia coli
50.8
-
wild-type AHAS I, 37°C, pH 7.6 Escherichia coli
60.4
-
wild-type AHAS II, 37°C, pH 7.6 Escherichia coli

Subunits

Subunits Comment Organism
More isozyme AHAS I, catalytic subunit ilvB, regulatory subunit ilvN. AHAS II, catalytic subunit ilvG, regulatory subunit ilvM. Isozyme AHAS III, catalytic subunit ilvI, regulatory subunit ilvH. AHAS II regulatory subunit ilvM is able to activate the catalytic subunits of all three of the isozymes, and the truncated AHAS III regulatory subunits ilvH-DELTA80, ilvH-DELTA86 and ilvH-DELTA89 are able to activate the catalytic subunits of both AHAS I and AHAS III. Contrary to wild-type, none of the heterologously activated enzymes have any feedback sensitivity Escherichia coli