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Literature summary for 2.2.1.6 extracted from

  • Loschonsky, S.; Waltzer, S.; Brecht, V.; Mueller, M.
    Elucidation of the enantioselective cyclohexane-1,2-dione hydrolase catalyzed formation of (S)-acetoin (2014), ChemCatChem, 6, 969-972.
No PubMed abstract available
Search for more literature for 2.2.1.6

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Azoarcus sp.

Organism

Organism UniProt Comment Textmining
Azoarcus sp.
-
-
-
Azoarcus sp. 22Lin
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 pyruvate enzymatic formation of highly enantioenriched acetoin from two molecules of pyruvate occurs without the release of acetaldehyde or acetolactate Azoarcus sp. (S)-acetoin + 2 CO2 87-90%ee ?
2 pyruvate enzymatic formation of highly enantioenriched acetoin from two molecules of pyruvate occurs without the release of acetaldehyde or acetolactate Azoarcus sp. 22Lin (S)-acetoin + 2 CO2 87-90%ee ?
additional information ThDP-dependent cyclohexane-1,2-dione hydrolase (CDH) is able to form (S)-acetoin with particularly high enantioselectivity (up to 95%ee) by all three possible pathways: homocoupling of pyruvate, homocoupling of acetaldehyde, or cross-coupling of acetaldehyde (as acceptor) and pyruvate (as donor), high enantioselectivity in the CDH-catalyzed formation of (S)-acetoin. An unprecedented non-acetolactate pathway for the homocoupling of pyruvate explains the high enantioselectivity in the CDH-catalyzed formation of (S)-acetoin, enzymatic formation of highly enantioenriched acetoin from two molecules of pyruvate occurs without the release of acetaldehyde or acetolactate, competition assay, mechanism, overview Azoarcus sp. ?
-
 ?
additional information ThDP-dependent cyclohexane-1,2-dione hydrolase (CDH) is able to form (S)-acetoin with particularly high enantioselectivity (up to 95%ee) by all three possible pathways: homocoupling of pyruvate, homocoupling of acetaldehyde, or cross-coupling of acetaldehyde (as acceptor) and pyruvate (as donor), high enantioselectivity in the CDH-catalyzed formation of (S)-acetoin. An unprecedented non-acetolactate pathway for the homocoupling of pyruvate explains the high enantioselectivity in the CDH-catalyzed formation of (S)-acetoin, enzymatic formation of highly enantioenriched acetoin from two molecules of pyruvate occurs without the release of acetaldehyde or acetolactate, competition assay, mechanism, overview Azoarcus sp. 22Lin ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Azoarcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Azoarcus sp.

Cofactor

Cofactor Comment Organism Structure
FAD one molecule per enzyme molecule Azoarcus sp.
thiamine diphosphate dependent on, one molecule per enzyme molecule Azoarcus sp.