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Literature summary for 2.2.1.7 extracted from
- Cloning and characterization of 1-deoxy-D-xylulose 5-phosphate synthase from Streptomyces sp. strain CL190, which uses both the mevalonate and nonmevalonate pathways for isopentenyl diphosphate biosynthesis (2000), J. Bacteriol., 182, 891-897.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Streptomyces sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | recombinant protein | Streptomyces sp. |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.065 | - |
pyruvate | recombinant protein | Streptomyces sp. | |
| 0.096 | - |
pyruvate | - |
Escherichia coli | |
| 0.12 | - |
D-glyceraldehyde 3-phosphate | recombinant protein | Streptomyces sp. | |
| 0.24 | - |
D-glyceraldehyde 3-phosphate | - |
Escherichia coli | |
| 35 | - |
D-glyceraldehyde | recombinant protein | Streptomyces sp. | |
| 38 | - |
D-glyceraldehyde | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli | |
| Mg2+ | recombinant protein, optimum concentration 1 mM | Streptomyces sp. | |
| Mn2+ | - |
Escherichia coli | |
| Mn2+ | recombinant protein, optimum concentration 1 mM | Streptomyces sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
2 * 60000, gel filtration | Escherichia coli |
| 65000 | 69000 | SDS-PAGE | Escherichia coli |
| 68000 | - |
predicted size from gene sequence | Streptomyces sp. |
| 70000 | - |
recombinant protein, SDS-PAGE | Streptomyces sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P77488 | - |
- |
| Streptomyces sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| overexpression | Escherichia coli |
| recombinant protein, expressed in Escherichia coli | Streptomyces sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + D-glyceraldehyde | - |
Streptomyces sp. | 1-deoxy-D-xylulose + CO2 | - |
? | |
| pyruvate + D-glyceraldehyde | - |
Escherichia coli | 1-deoxy-D-xylulose + CO2 | - |
? | |
| pyruvate + D-glyceraldehyde 3-phosphate | - |
Streptomyces sp. | 1-deoxy-D-xylulose 5-phosphate + CO2 | - |
? | |
| pyruvate + D-glyceraldehyde 3-phosphate | - |
Escherichia coli | 1-deoxy-D-xylulose 5-phosphate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 65000-70000, gel filtration, native PAGE | Streptomyces sp. |
| dimer | 2 * 60000, gel filtration | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | 44 | - |
Escherichia coli |
| 42 | 44 | recombinant protein | Streptomyces sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Escherichia coli |
| 9 | - |
recombinant protein | Streptomyces sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | failure of DXP producting in the absence of thiamine diphosphate indicates its absolute requirement for the enzymatic reaction | Streptomyces sp. | |
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