Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.1 extracted from

  • Shigesada, K.; Tatibana, M.
    N-Acetylglutamate synthetase from rat-liver mitochondria. Partial purification and catalytic properties (1978), Eur. J. Biochem., 84, 285-291.
    View publication on PubMed
Search for more literature for 2.3.1.1

Activating Compound

Activating Compound Comment Organism Structure
arginine
-
 Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) strong inhibition Rattus norvegicus
AgNO3 0.1 mM, 70-90% inhibition Rattus norvegicus
BaCl2 1 mM, 30-50% inhibition Rattus norvegicus
CaCl2 1 mM, 30-50% inhibition Rattus norvegicus
CoCl2 0.1 mM, 30-50% inhibition Rattus norvegicus
coenzyme A
-
 Rattus norvegicus
Cu2+ CuSO4, 0.1 mM, 70-90% inhibition Rattus norvegicus
EDTA weak inhibition Rattus norvegicus
FeCl3 0.1 mM, 30-50% inhibition Rattus norvegicus
FeSO4 0.1 mM, 30-50% inhibition Rattus norvegicus
Hg2+ Hg(CHCOO)2, 0.1 mM, 70-90% inhibition Rattus norvegicus
K2SO4
-
 Rattus norvegicus
KCl 100 mM, 30% inhibition, 200 mM, 50% inhibition Rattus norvegicus
L-alpha-Acetoxylglutamate 2 mM, 17% inhibition Rattus norvegicus
MgCl2 1 mM, 30-50% inhibition Rattus norvegicus
MnCl2 0.1 mM, 30-50% inhibition Rattus norvegicus
N-acetyl-D-glutamate 2 mM, 30% inhibition Rattus norvegicus
N-acetyl-DL-alpha-aminoadipate 2 mM, 78% inhibition Rattus norvegicus
N-acetyl-L-aspartate 2 mM, 25% inhibition Rattus norvegicus
N-acetyl-L-glutamine 2 mM, 46% inhibition Rattus norvegicus
N-acetylglutamate
-
 Rattus norvegicus
N-benzoyl-L-glutamate 2 mM, 29% inhibition Rattus norvegicus
N-Butyryl-L-glutamate 2 mM, 19% inhibition Rattus norvegicus
N-carbamoyl-L-glutamate 2 mM, 31% inhibition Rattus norvegicus
N-propionyl-L-glutamate 2 mM, 63% inhibition Rattus norvegicus
Ni(NO3)2 0.1 mM, 30-50% inhibition Rattus norvegicus
p-hydroxymercuribenzoate strong inhibition Rattus norvegicus
Pb(NO3)2 0.1 mM, 70-90% inhibition Rattus norvegicus
potassium phosphate
-
 Rattus norvegicus
succinate 2 mM, 21% inhibition Rattus norvegicus
Zn2+ ZnCl2, 0.1 mM, 70-90% inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 acetyl-CoA
-
 Rattus norvegicus
3
-
 L-glutamate
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000 300000 gel filtration, mixture of multiple forms Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, hydroxyapatite, DEAE-cellulose, Sephadex G-100 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.012
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, 100 mM phosphate buffer, pH 7.5, 6 months, 50% loss of activity Rattus norvegicus
4°C, 100 mM potassium phosphate buffer, pH 7.5, 24 h, 10% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + glycine
-
 Rattus norvegicus CoA + acetylaminoacetate
-
 ?
acetyl-CoA + L-glutamate
-
 Rattus norvegicus CoA + N-acetyl-L-glutamate
-
 ?
acetyl-CoA + L-glutamine
-
 Rattus norvegicus ?
-
 ?
benzoyl-CoA + L-glutamate very low activity Rattus norvegicus CoA + N-benzoyl-L-glutamate
-
 ?
butyryl-CoA + L-glutamate very low activity Rattus norvegicus CoA + N-butyryl-L-glutamate
-
 ?
propionyl-CoA + L-glutamate
-
 Rattus norvegicus CoA + N-propionyl-L-glutamate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 liver enzyme, in the absence of arginine Rattus norvegicus
8
-
 in the absence of arginine Rattus norvegicus
8.5
-
 in the presence of arginine Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.07
-
 acetyl-glutamate
-
 Rattus norvegicus