KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
formylmethanofuran | - |
Methanosarcina barkeri | |
0.04 | - |
5,6,7,8-tetrahydromethanopterin | - |
Methanosarcina barkeri | |
0.05 | - |
formylmethanofuran | - |
Methanothermobacter thermautotrophicus | |
0.06 | - |
formylmethanofuran | - |
Methanosarcina barkeri | |
0.06 | - |
formylmethanofuran | - |
Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
gel filtration | Methanosarcina barkeri |
32000 | - |
1 * 32000 SDS-PAGE | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | Methanosarcina barkeri | involved in the formation of methane from CO2 | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | Methanothermobacter thermautotrophicus | involved in the formation of methane from CO2 | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | - |
- |
- |
Methanothermobacter thermautotrophicus | - |
- |
- |
Oxidation Stability | Organism |
---|---|
stable in presence of molecular oxygen | Methanosarcina barkeri |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina barkeri |
- |
Methanothermobacter thermautotrophicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin | ternary complex type mechanism | Methanosarcina barkeri | |
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin | ternary complex type mechanism | Methanothermobacter thermautotrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
610 | - |
- |
Methanosarcina barkeri |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tricine/KOH, pH 8, stable for 1 week | Methanosarcina barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | - |
Methanosarcina barkeri | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | - |
Methanothermobacter thermautotrophicus | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | involved in the formation of methane from CO2 | Methanosarcina barkeri | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | involved in the formation of methane from CO2 | Methanothermobacter thermautotrophicus | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 32000 SDS-PAGE | Methanosarcina barkeri |
monomer | 1 * 32000 SDS-PAGE | Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Methanosarcina barkeri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
stable for more than 4 h | Methanosarcina barkeri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1970 | - |
5,6,7,8-tetrahydromethanopterin | - |
Methanosarcina barkeri | |
2770 | - |
5,6,7,8-tetrahydromethanopterin | - |
Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Methanosarcina barkeri |