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Literature summary for 2.3.1.101 extracted from
- Characterization of the formyltransferase from Methylobacterium extorquens AM1 (2001), Eur. J. Biochem., 268, 4769-4775.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Methylorubrum extorquens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
5,6,7,8-tetrahydromethanopterin | - |
Methylorubrum extorquens |  |
| 0.05 | - |
formylmethanofuran | - |
Methylorubrum extorquens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | 1 M increases the activity 20fold | Methylorubrum extorquens | |
| K2HPO4 | 1.5 M stimulates 10fold | Methylorubrum extorquens | |
| additional information | not: Fe, Mo, V, Wo | Methylorubrum extorquens | |
| NaCl | 1 M increases the activity 20fold | Methylorubrum extorquens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 310000 | - |
enzyme complex with three other polypeptides that shows sequence identities with the subunits of formylmethanofuran-dehydrogenase and are required for stability of enzyme, the subunits are present in 2:2:2:2 stoichiometry | Methylorubrum extorquens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | Methylorubrum extorquens | - |
methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
r | |
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | - |
methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylorubrum extorquens | - |
gene ffsA | - |
| Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | - |
gene ffsA | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| if a single chromatographic step was performed 90% loss of activity, in 24 h 100% loss of activity | Methylorubrum extorquens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of enzyme complex | Methylorubrum extorquens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin | pathway via enzyme complex | Methylorubrum extorquens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Methylorubrum extorquens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | - |
Methylorubrum extorquens | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
r | |
| formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | - |
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | enzyme is the 32000 Da subunit of enzyme complex | Methylorubrum extorquens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Methylorubrum extorquens |
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