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Literature summary for 2.3.1.101 extracted from

  • Shima, S.; Herault, D.A.; Berkessel, A.; Thauer, R.K.
    Activation and thermostabilization effects of cyclic 2,3-diphosphoglycerate on enzymes from the hyperthermophilic Methanopyrus kandleri (1998), Arch. Microbiol., 170, 469-472.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2,3-diphosphoglycerate the 1 M 2,3-diphosphoglycerate the enzyme is highly active Methanopyrus kandleri

General Stability

General Stability Organism
at cyclic 2,3-diphosphoglycerate concentrations prevailing in the cells of Methanopyrus kandleri the enzyme is completely thermostable. At molar concentrations also the potassium salts of phosphate and of 2,3-bisphosphoglycerate, the biosynthetic precursor of cyclic 2,3-diphosphoglycerate confer thermostability to the enzymes Methanopyrus kandleri

Organism

Organism UniProt Comment Textmining
Methanopyrus kandleri
-
-
-

Oxidation Stability

Oxidation Stability Organism
stable under oxic conditions Methanopyrus kandleri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
-
Methanopyrus kandleri methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
-
?

Synonyms

Synonyms Comment Organism
formylmethanofuran:tetrahydromethanopterin formyltransferase
-
Methanopyrus kandleri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
assay at Methanopyrus kandleri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
at cyclic 2,3-diphosphoglycerate concentrations prevailing in the cells of Methanopyrus kandleri the enzyme is completely thermostable. At molar concentrations also the potassium salts of phosphate and of 2,3-bisphosphoglycerate, the biosynthetic precursor of cyclic 2,3-diphosphoglycerate confer thermostability to the enzymes Methanopyrus kandleri
90
-
in 0.5 M 2,3-diphosphoglycerate, the cyclohydrolase is completely stable at pH 8.0 and 90°C. Methanopyrus kandleri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Methanopyrus kandleri