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Literature summary for 2.3.1.108 extracted from

  • Castro-Castro, A.; Janke, C.; Montagnac, G.; Paul-Gilloteaux, P.; Chavrier, P.
    ATAT1/MEC-17 acetyltransferase and HDAC6 deacetylase control a balance of acetylation of alpha-tubulin and cortactin and regulate MT1-MMP trafficking and breast tumor cell invasion (2012), Eur. J. Cell Biol., 91, 950-960.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
ATAT1, DNA and amino acid sequence determination and analysis, stable functional expression of EGFP-ATAT1 and mutant ATAT1D157N in MDA-MB-231 cells and in HeLa cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D157N site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
additional information overexpression of ATAT1 phenocopies the effect of HDAC6 inhibition by trichostatin Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + cortactin Homo sapiens ATAT1 acetylates, binds and colocalizes with cortactin at the adherent surface of MDA-MB-231 cells CoA + N-acetyl-cortactin
-
?
acetyl-CoA + [alpha-tubulin]-L-lysine Homo sapiens acetylation of alpha-tubulin mostly occurs on lysine residue 40, which is localized in the microtubule lumen CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
MDA-MB-231 cell invasive breast cancer cells Homo sapiens
-
additional information ATAT1 colocalizes with cortactin at the adherent surface of the cells, association of ATAT1 and cortactin at invadopodia, subcellular localization study, overview Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + cortactin
-
Homo sapiens CoA + N-acetyl-cortactin
-
?
acetyl-CoA + cortactin ATAT1 acetylates, binds and colocalizes with cortactin at the adherent surface of MDA-MB-231 cells Homo sapiens CoA + N-acetyl-cortactin
-
?
acetyl-CoA + [alpha-tubulin]-L-lysine
-
Homo sapiens CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
?
acetyl-CoA + [alpha-tubulin]-L-lysine acetylation of alpha-tubulin mostly occurs on lysine residue 40, which is localized in the microtubule lumen Homo sapiens CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
?

Synonyms

Synonyms Comment Organism
alpha-tubulin N-acetyltransferase 1
-
Homo sapiens
ATAT1
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Homo sapiens
ATAT1 acetylase
-
Homo sapiens
ATAT1 tubulin acetyltransferase
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
Homo sapiens

General Information

General Information Comment Organism
malfunction overexpression of ATAT1 phenocopies the effect of HDAC6 inhibition by trichostatin. Random invasive migration of cells in 3D collagen I is inhibited by 40-50% upon MT1-MMP or ATAT1 knockdown Homo sapiens
metabolism a balance of acetylation and deaceylation by ATAT1/HDAC6, histone deacetylase 6, enzymes with opposite activities regulates the migratory and invasive capacities of breast tumor cells Homo sapiens
physiological function dynamics and distribution of MT1-MMP-positive endosomes require regulation of acetylation levels, ATAT1 tubulin acetyltransferase binds and regulates cortactin acetylation levels. Acetylation of alpha-tubulin mostly occurs on lysine residue 40, which is localized in the microtubule lumen. ATAT1 colocalizes with cortactin at the adherent surface of the cells and it is required for 2D migration and invasive migration of MDA-MB-231 cells in collagen matrix Homo sapiens