General Stability | Organism |
---|---|
10% glycerol in liquid N2 stabilizes | Nicotiana tabacum |
freezing does not alter the properties of the enzyme | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | at 1 mM, 92% inhibition | Nicotiana tabacum | |
p-chloromercuribenzoate | 1 mM, strong | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0049 | - |
feruloyl-CoA | + tyramine | Nicotiana tabacum | |
0.025 | - |
tyramine | + feruloyl-CoA, dopamine + feruloyl-CoA | Nicotiana tabacum | |
0.045 | 0.07 | tyramine | + p-coumaroyl-CoA, depending on the enzyme fraction used | Nicotiana tabacum | |
0.0588 | 0.071 | tyramine | + feruloyl-CoA, depending on the enzyme fraction used | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
feruloyl-CoA + tyramine | Nicotiana tabacum | - |
CoA + feruloyltyramine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
infected with tobacco mosaic virus | - |
Oxidation Stability | Organism |
---|---|
O2-sensitive | Nicotiana tabacum |
Purification (Comment) | Organism |
---|---|
46fold, separation of 3 fractions with different specificities | Nicotiana tabacum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana tabacum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0156 | - |
- |
Nicotiana tabacum |
Storage Stability | Organism |
---|---|
4°C, as ammonium sulfate paste, 10 mM mercaptoethanol, 24 h, 25% loss of activity | Nicotiana tabacum |
4°C, as ammonium sulfate paste, 5 mM DTT, 10% loss of activity within 24 h | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + tyramine | - |
Nicotiana tabacum | CoA + N-(4-coumaroyl)tyramine | i.e. 4-hydroxycinnamoyltyramine | ? | |
cinnamoyl-CoA + tyramine | - |
Nicotiana tabacum | CoA + cinnamoyltyramine | - |
? | |
feruloyl-CoA + dopamine | - |
Nicotiana tabacum | CoA + feruloyldopamine | - |
? | |
feruloyl-CoA + tyramine | - |
Nicotiana tabacum | CoA + feruloyltyramine | - |
? | |
additional information | enzyme has a wide specificity for phenylethylamines and cinnamoyl-CoA thioesters but differs in the affinity for the substrate, thus indicating different isoenzymes | Nicotiana tabacum | ? | - |
? | |
sinapoyl-CoA + tyramine | - |
Nicotiana tabacum | CoA + sinapoyltyramine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Nicotiana tabacum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | 8 | optimum varies slightly between different fractions | Nicotiana tabacum |