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Literature summary for 2.3.1.116 extracted from

  • Suzuki, H.; Nakayama, T.; Nagae, S.; Yamaguchi, M.A.; Iwashita, T.; Fukui, Y.; Nishino, T.
    cDNA cloning and functional characterization of flavonol 3-O-glucoside-6''-O-malonyltransferases from flowers of Verbena hybrida and Lamium purpureum (2004), J. Mol. Catal. B, 28, 87-93.
No PubMed abstract available
Search for more literature for 2.3.1.116

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Glandularia x hybrida
expression in Escherichia coli Lamium purpureum

Organism

Organism UniProt Comment Textmining
Glandularia x hybrida
-
-
-
Lamium purpureum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Glandularia x hybrida
-
flower
-
 Lamium purpureum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + genistein 7-O-glucoside 7.6% of the activity with quercetin 3-O-glucoside Glandularia x hybrida CoA + ?
-
 ?
malonyl-CoA + p-nitrophenyl beta-D-glucopyranoside 19% of the activity with quercetin 3-O-glucoside Lamium purpureum CoA + p-nitrophenyl 6-O-malonyl-beta-D-glucopyranoside
-
 ?
malonyl-CoA + p-nitrophenyl beta-D-glucopyranoside 48% of the activity with quercetin 3-O-glucoside Glandularia x hybrida CoA + p-nitrophenyl 6-O-malonyl-beta-D-glucopyranoside
-
 ?
malonyl-CoA + phenyl beta-D-glucopyranoside 11% of the activity with quercetin 3-O-glucoside Lamium purpureum CoA + phenyl 6-O-malonyl-beta-D-glucopyranoside
-
 ?
malonyl-CoA + phenyl beta-D-glucopyranoside 6.4% of the activity with quercetin 3-O-glucoside Glandularia x hybrida CoA + phenyl 6-O-malonyl-beta-D-glucopyranoside
-
 ?
malonyl-CoA + quercetin 3-O-glucoside regiospecific transfer of malonyl group to the 6’’-hydroxyl group of quercetin 3-O-glucoside, highly specific for these acyl donor and acceptor Lamium purpureum CoA + quercetin 3-O-(6-O-malonyl-beta-D-glucoside)
-
 ?
malonyl-CoA + quercetin 3-O-glucoside regiospecific transfer of malonyl group to the 6’’-hydroxyl group of quercetin 3-O-glucoside, highly specific for these acyl donor and acceptor Glandularia x hybrida CoA + quercetin 3-O-(6-O-malonylbeta-D-glucoside)
-
 ?
additional information less than 2% of the activity with quercetin 3-O-glucoside: pelargonidine, cyanidin, delphinidin, daidzein, genistein, o-(hydroxymethyl)phenyl beta-D-glucopyranoside, less than 2% of the activity with malonyl-CoA: acetyl-CoA, methylmalonyl-CoA Lamium purpureum ?
-
 ?
additional information less than 2% of the activity with quercetin 3-O-glucoside: pelargonidine, cyanidin, delphinidin, daidzein, o-(hydroxymethyl)phenyl beta-D-glucopyranoside, less than 2% of the activity with malonyl-CoA: acetyl-CoA, methylmalonyl-CoA Glandularia x hybrida ?
-
 ?
succinyl-CoA + quercetin 3-O-glucoside 27% of the activity with malonyl-CoA Lamium purpureum CoA + quercetin 3-(6-O-succinylbeta-D-glucoside)
-
 ?
succinyl-CoA + quercetin 3-O-glucoside 19% of the activity with malonyl-CoA Glandularia x hybrida CoA + quercetin 3-O-(6-O-succinyl-beta-D-glucoside)
-
 ?

Synonyms

Synonyms Comment Organism
MaT1
-
 Glandularia x hybrida
MaT1
-
 Lamium purpureum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.9
-
 malonyl-CoA pH 7.0, 30°C Glandularia x hybrida
2.9
-
 quercetin 3-O-glucoside pH 7.0, 30°C Glandularia x hybrida
9
-
 malonyl-CoA pH 7.0, 30°C Lamium purpureum
9
-
 quercetin 3-O-glucoside pH 7.0, 30°C Lamium purpureum