Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
His9-tagged catalytic domain, hanging drop vapor diffusion method, using 0.2 M potassium thiocyanate and 20% (w/v) polyethylene glycol 3350 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H602C | inactive | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30028 | - |
x * 30028, catalytic domain, calculated from amino acid sequence | Escherichia coli |
30030 | - |
x * 30030, catalytic domain, FT mass spectrometry | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine | Escherichia coli | - |
CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P06959 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity resin column chromatography, Superdex G75 gel filtration, and G3000SW TSK gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine | - |
Escherichia coli | CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine | - |
r | |
CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine | - |
Escherichia coli | acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 30028, catalytic domain, calculated from amino acid sequence | Escherichia coli |
? | x * 30030, catalytic domain, FT mass spectrometry | Escherichia coli |
trimer | x-ray crystallography | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
dihydrolipoamide acetyltransferase | - |
Escherichia coli |
pyruvate dehydrogenase complex component E2p | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Escherichia coli |