General Stability | Organism |
---|---|
bovine serum albumin prevents denaturation | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | 0.5 M | Escherichia coli | |
LiCl | 0.5 M | Escherichia coli | |
additional information | no inhibition by N-ethylmaleimide, UDP-2,3-diacyl-N-glucosamine, lipid X, lipid IVA, 3-deoxy-D-mannooctulosonate, octyl-beta-D-glucoside | Escherichia coli | |
myristoyl-[acyl-carrier protein] | - |
Escherichia coli | |
NaCl | 0.5 M | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] | - |
Escherichia coli | |
0.099 | - |
UDP-N-acetylglucosamine | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | slight activation at 0.25 M | Escherichia coli | |
LiCl | slight activation at 0.25 M | Escherichia coli | |
NaCl | slight activation at 0.25 M | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
x * 27000, calculated from nucleotide sequence | Escherichia coli |
30000 | - |
alpha2, 2 * 30000, SDS-PAGE | Escherichia coli |
30000 | - |
alpha3, 3 * 30000, SDS-PAGE | Escherichia coli |
90000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
MC1061/pSR1 (overproducing strain harbouring plasmid pSR1) | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.3 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | no substrates are UDPglucosamine, UDP-N-(R)-3-hydroxymyristoylglucosamine, ADP-N-acetylglucosamine, GDP-N-acetylglucosamine, CDP-N-acetylglucosamine, (R)-3-hydroxytetradecanoyl-CoA, or palmitoyl-CoA, myristoyl-[acyl-carrier-protein], and palmitoyl-[acyl-carrier-protein] | Escherichia coli | [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
r | |
(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | high specificity with respect to acyl-donor, equilibrium constant favors thioester acyl carrier substrate | Escherichia coli | [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 27000, calculated from nucleotide sequence | Escherichia coli |
dimer or trimer | alpha2, 2 * 30000, SDS-PAGE | Escherichia coli |
trimer | alpha3, 3 * 30000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.2 | broad | Escherichia coli |