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Literature summary for 2.3.1.129 extracted from
- The active site of Escherichia coli UDP-N-acetylglucosamine acyltransferase. Chemical modification and site-directed mutagenesis (1999), J. Biol. Chem., 274, 27047-27055.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H122A | lower specific activity than wild-type | Escherichia coli |
| H122N | lower specific activity than wild-type | Escherichia coli |
| H125A | lower specific activity than wild-type | Escherichia coli |
| H125N | lower specific activity than wild-type | Escherichia coli |
| H144A | lower specific activity than wild-type | Escherichia coli |
| H144N | lower specific activity than wild-type | Escherichia coli |
| H160A | lower specific activity than wild-type | Escherichia coli |
| H160F | lower specific activity than wild-type | Escherichia coli |
| K76A | lower specific activity than wild-type | Escherichia coli |
| K76R | lower specific activity than wild-type | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyldicarbonate | - |
Escherichia coli |  |
| Phenylglyoxal | - |
Escherichia coli | |
| pyridoxal 5'-phosphate/sodium borohydride | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.82 | - |
UDP-N-acetylglucosamine | wild-type | Escherichia coli | |
| 7 | - |
UDP-N-acetylglucosamine | K76R | Escherichia coli | |
| 15 | - |
UDP-N-acetylglucosamine | K76A | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | - |
Escherichia coli | [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.17 | - |
UDP-N-acetylglucosamine | wild-type | Escherichia coli | |
| 9.83 | - |
UDP-N-acetylglucosamine | K76R | Escherichia coli | |
| 10.5 | - |
UDP-N-acetylglucosamine | K76A | Escherichia coli | |
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Release 2023.1 (January 2023)
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