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Literature summary for 2.3.1.130 extracted from
- Enzymatic synthesis of hydroxycinnamic acid esters of sugar acids and hydroaromatic acids by protein preparations from rye (Secale cereale) primary leaves (1987), J. Plant Physiol., 131, 61-73.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DTT | activation | Secale cereale |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.045 | - |
feruloyl-CoA | - |
Secale cereale | |
| 33 | - |
galactarate | - |
Secale cereale | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Secale cereale | - |
rye, var. Kustro | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Secale cereale |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | primary | Secale cereale | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0033 | - |
- |
Secale cereale |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, at least 6 months | Secale cereale |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| caffeoyl-CoA + galactarate | poor substrate | Secale cereale | CoA + O-caffeoylgalactarate | - |
? | |
| feruloyl-CoA + galactarate | best substrate, no substrates are 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate | Secale cereale | CoA + O-feruloylgalactarate | - |
? | |
| p-coumaroyl-CoA + galactarate | condensation at 69% the rate of the reaction with feruloyl-CoA | Secale cereale | CoA + O-p-coumaroylgalactarate | - |
? | |
| sinapoyl-CoA + galactarate | condensation at 78% the rate of the reaction with feruloyl-CoA | Secale cereale | CoA + O-sinapoylgalactarate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Secale cereale |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
denaturation after 5 min | Secale cereale |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
25 mM potassium phosphate buffer | Secale cereale |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | 7.6 | about 80% of maximal activity at pH 7.1 and 7.6 | Secale cereale |
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