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Literature summary for 2.3.1.133 extracted from

  • Strack, D.; Keller, H.; Weissenböck, G.
    Enzymatic synthesis of hydroxycinnamic acid esters of sugar acids and hydroaromatic acids by protein preparations from rye (Secale cereale) primary leaves (1987), J. Plant Physiol., 131, 61-73.
No PubMed abstract available
Search for more literature for 2.3.1.133

Activating Compound

Activating Compound Comment Organism Structure
EDTA activation Secale cereale

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 4-coumaroyl-CoA
-
 Secale cereale
0.045
-
 caffeoyl-CoA
-
 Secale cereale
0.59
-
 shikimate with 4-coumaroyl-CoA Secale cereale
83
-
 shikimate with caffeoyl-CoA Secale cereale

Organism

Organism UniProt Comment Textmining
Secale cereale
-
 rye, var. Kustro
-

Purification (Commentary)

Purification (Comment) Organism
partial Secale cereale

Source Tissue

Source Tissue Comment Organism Textmining
leaf primary Secale cereale
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0056
-
-
 Secale cereale

Storage Stability

Storage Stability Organism
-20°C, at least 6 months Secale cereale

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + shikimate best substrate Secale cereale CoA + 5-O-(4-coumaroyl)shikimate
-
 ?
caffeoyl-CoA + shikimate condensation at 52% the rate of the reaction with p-coumaroyl-CoA Secale cereale CoA + 5-O-caffeoylshikimate
-
 ?
feruloyl-CoA + shikimate condensation at 22% the rate of the reaction with p-coumaroyl-CoA Secale cereale CoA + 5-O-feruloylshikimate
-
 ?
additional information 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate Secale cereale ?
-
 ?
sinapoyl-CoA + shikimate condensation at 29% the rate of the reaction with p-coumaroyl-CoA Secale cereale CoA + 5-O-sinapoylshikimate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 denaturation after 5 min Secale cereale

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
 200 mM potassium phosphate buffer Secale cereale

pH Range

pH Minimum pH Maximum Comment Organism
6.3 7.7 about 80% of maximal activity at pH 6.3 and 7.7 Secale cereale