Cloned (Comment) | Organism |
---|---|
expression of GST-tagged HCT in Escherichia coli strain BL21 (DE3) | Populus x canadensis |
genes PeHCT-1, PeHCT-2, PeHCT-3, PeHCT-4, PeHCT-5, PeHCT-6, and PeHCT-7, expression as GST-tagged enzymes in Escherichia coli strain BL21 (DE3) | Populus x canadensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, overview | Populus x canadensis | |
0.0009 | - |
shikimate | pH 8.0, 37°C, recombinant GST-tagged enzyme, with 4-coumaroyl-CoA | Populus x canadensis | |
0.00582 | - |
quinate | pH 8.0, 37°C, recombinant GST-tagged enzyme, with 4-coumaroyl-CoA | Populus x canadensis | |
0.00813 | - |
4-coumaroyl-CoA | pH 8.0, 37°C, recombinant GST-tagged enzyme, with shikimate | Populus x canadensis | |
0.00964 | - |
4-coumaroyl-CoA | pH 8.0, 37°C, recombinant GST-tagged enzyme, with quinate | Populus x canadensis | |
0.9 | - |
shikimate | with 4-coumaroyl-CoA, pH 8.0, 37°C | Populus x canadensis | |
5.82 | - |
quinate | with 4-coumaroyl-CoA, pH 8.0, 37°C | Populus x canadensis | |
8.13 | - |
4-coumaroyl-CoA | with shikimate, pH 8.0, 37°C | Populus x canadensis | |
9.64 | - |
4-coumaroyl-CoA | with quinate, pH 8.0, 37°C | Populus x canadensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, about, recombinant GST-tagged HCT, SDS-PAGE | Populus x canadensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + quinate | Populus x canadensis | - |
CoA + 5-(4-coumaroyl)quinate | - |
? | |
4-coumaroyl-CoA + shikimate | Populus x canadensis | recombinant HCT shows substrate-specificity towards shikimic acid in contrast to hydroxycinnamoyl-CoA quinate transferase, HQT, EC 2.3.1.99 | CoA + 4-coumaroylshikimate | - |
? | |
additional information | Populus x canadensis | HCT mediates formation of hydroxycinnamoylquinnate or hydroxycinnamoylshikimate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus x canadensis | - |
- |
- |
Populus x canadensis | - |
genes PeHCT-1, PeHCT-2, PeHCT-3, PeHCT-4, PeHCT-5, PeHCT-6, and PeHCT-7 | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged HCT from Escherichia coli strain BL21 (DE3) by glutathione affinity chromatography | Populus x canadensis |
recombinant GST-tagged PeHCT-1, PeHCT-2, PeHCT-3, PeHCT-4, PeHCT-5, PeHCT-6, and PeHCT-7 gene products from Escherichia coli strain BL21 (DE3) by glutathione affinity cromatography | Populus x canadensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Populus x canadensis | - |
additional information | PeHCT displays 1.5fold higher expression in the stem than in the leaf, and its expression increases during the growth stage of the leaf | Populus x canadensis | - |
petiole | - |
Populus x canadensis | - |
stem | - |
Populus x canadensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + quinate | preferred substrate is shikimate | Populus x canadensis | CoA + 4-coumaroylquinate | - |
? | |
4-coumaroyl-CoA + quinate | - |
Populus x canadensis | CoA + 5-(4-coumaroyl)quinate | - |
? | |
4-coumaroyl-CoA + shikimate | recombinant HCT shows substrate-specificity towards shikimic acid in contrast to hydroxycinnamoyl-CoA quinate transferase, HQT, EC 2.3.1.99 | Populus x canadensis | CoA + 4-coumaroylshikimate | - |
? | |
4-coumaroyl-CoA + shikimate | preferred substrate is shikimate | Populus x canadensis | CoA + 4-coumaroylshikimate | - |
? | |
additional information | HCT mediates formation of hydroxycinnamoylquinnate or hydroxycinnamoylshikimate | Populus x canadensis | ? | - |
? | |
additional information | in vitro recombinant PeHCT shows substrate-specificity towards shikimic acid | Populus x canadensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, about, recombinant GST-tagged HCT, SDS-PAGE | Populus x canadensis |
Synonyms | Comment | Organism |
---|---|---|
HCT | - |
Populus x canadensis |
hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyltransferase | - |
Populus x canadensis |
shikimate hydroxycinnamoyltransferase | - |
Populus x canadensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Populus x canadensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Populus x canadensis |
General Information | Comment | Organism |
---|---|---|
evolution | HCT is a member of the BAHD [BEAT (benzylalcohol O-acetyltransferase)] family acyltransferases | Populus x canadensis |
evolution | HCT is a member of the BAHD, i.e. BEAT (benzylalcohol O-acetyltransferase), family acyltransferases | Populus x canadensis |
metabolism | HCT is likely to be involved in lignin biosynthesis, overview | Populus x canadensis |
metabolism | PeHCT might be involved in lignin biosynthesis by formation of 4-coumaroyl shikimate | Populus x canadensis |
additional information | PeHCT contains a conserved HXXXDG motif that is found in acyltransferase | Populus x canadensis |