Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.133 extracted from

  • Kim, B.; Kim, I.; Ahn, J.
    Characterization of hydroxycinnamoyl-coenzyme A shikimate hydroxycinnamoyltransferase from Populus euramericana (2011), J. Korean Soc. Appl. Biol. Chem., 54, 817-821.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-tagged HCT in Escherichia coli strain BL21 (DE3) Populus x canadensis
genes PeHCT-1, PeHCT-2, PeHCT-3, PeHCT-4, PeHCT-5, PeHCT-6, and PeHCT-7, expression as GST-tagged enzymes in Escherichia coli strain BL21 (DE3) Populus x canadensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics, overview Populus x canadensis
0.0009
-
shikimate pH 8.0, 37°C, recombinant GST-tagged enzyme, with 4-coumaroyl-CoA Populus x canadensis
0.00582
-
quinate pH 8.0, 37°C, recombinant GST-tagged enzyme, with 4-coumaroyl-CoA Populus x canadensis
0.00813
-
4-coumaroyl-CoA pH 8.0, 37°C, recombinant GST-tagged enzyme, with shikimate Populus x canadensis
0.00964
-
4-coumaroyl-CoA pH 8.0, 37°C, recombinant GST-tagged enzyme, with quinate Populus x canadensis
0.9
-
shikimate with 4-coumaroyl-CoA, pH 8.0, 37°C Populus x canadensis
5.82
-
quinate with 4-coumaroyl-CoA, pH 8.0, 37°C Populus x canadensis
8.13
-
4-coumaroyl-CoA with shikimate, pH 8.0, 37°C Populus x canadensis
9.64
-
4-coumaroyl-CoA with quinate, pH 8.0, 37°C Populus x canadensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, about, recombinant GST-tagged HCT, SDS-PAGE Populus x canadensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-coumaroyl-CoA + quinate Populus x canadensis
-
CoA + 5-(4-coumaroyl)quinate
-
?
4-coumaroyl-CoA + shikimate Populus x canadensis recombinant HCT shows substrate-specificity towards shikimic acid in contrast to hydroxycinnamoyl-CoA quinate transferase, HQT, EC 2.3.1.99 CoA + 4-coumaroylshikimate
-
?
additional information Populus x canadensis HCT mediates formation of hydroxycinnamoylquinnate or hydroxycinnamoylshikimate ?
-
?

Organism

Organism UniProt Comment Textmining
Populus x canadensis
-
-
-
Populus x canadensis
-
genes PeHCT-1, PeHCT-2, PeHCT-3, PeHCT-4, PeHCT-5, PeHCT-6, and PeHCT-7
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged HCT from Escherichia coli strain BL21 (DE3) by glutathione affinity chromatography Populus x canadensis
recombinant GST-tagged PeHCT-1, PeHCT-2, PeHCT-3, PeHCT-4, PeHCT-5, PeHCT-6, and PeHCT-7 gene products from Escherichia coli strain BL21 (DE3) by glutathione affinity cromatography Populus x canadensis

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Populus x canadensis
-
additional information PeHCT displays 1.5fold higher expression in the stem than in the leaf, and its expression increases during the growth stage of the leaf Populus x canadensis
-
petiole
-
Populus x canadensis
-
stem
-
Populus x canadensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + quinate preferred substrate is shikimate Populus x canadensis CoA + 4-coumaroylquinate
-
?
4-coumaroyl-CoA + quinate
-
Populus x canadensis CoA + 5-(4-coumaroyl)quinate
-
?
4-coumaroyl-CoA + shikimate recombinant HCT shows substrate-specificity towards shikimic acid in contrast to hydroxycinnamoyl-CoA quinate transferase, HQT, EC 2.3.1.99 Populus x canadensis CoA + 4-coumaroylshikimate
-
?
4-coumaroyl-CoA + shikimate preferred substrate is shikimate Populus x canadensis CoA + 4-coumaroylshikimate
-
?
additional information HCT mediates formation of hydroxycinnamoylquinnate or hydroxycinnamoylshikimate Populus x canadensis ?
-
?
additional information in vitro recombinant PeHCT shows substrate-specificity towards shikimic acid Populus x canadensis ?
-
?

Subunits

Subunits Comment Organism
? x * 70000, about, recombinant GST-tagged HCT, SDS-PAGE Populus x canadensis

Synonyms

Synonyms Comment Organism
HCT
-
Populus x canadensis
hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyltransferase
-
Populus x canadensis
shikimate hydroxycinnamoyltransferase
-
Populus x canadensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Populus x canadensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Populus x canadensis

General Information

General Information Comment Organism
evolution HCT is a member of the BAHD [BEAT (benzylalcohol O-acetyltransferase)] family acyltransferases Populus x canadensis
evolution HCT is a member of the BAHD, i.e. BEAT (benzylalcohol O-acetyltransferase), family acyltransferases Populus x canadensis
metabolism HCT is likely to be involved in lignin biosynthesis, overview Populus x canadensis
metabolism PeHCT might be involved in lignin biosynthesis by formation of 4-coumaroyl shikimate Populus x canadensis
additional information PeHCT contains a conserved HXXXDG motif that is found in acyltransferase Populus x canadensis