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Literature summary for 2.3.1.133 extracted from

  • Sander, M.; Petersen, M.
    Distinct substrate specificities and unusual substrate flexibilities of two hydroxycinnamoyltransferases, rosmarinic acid synthase and hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyl-transferase, from Coleus blumei Benth. (2011), Planta, 233, 1157-1171.
    View publication on PubMed
Search for more literature for 2.3.1.133

Cloned(Commentary)

Cloned (Comment) Organism
gene cbhct2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged HST in Escherichia coli strain BL21(DE3)pLysS Coleus scutellarioides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, overview Coleus scutellarioides
0.0015
-
 4-coumaroyl-CoA pH 7.0, 30°C, with 3-hydroxybenzoate Coleus scutellarioides
0.0031
-
 4-coumaroyl-CoA pH 7.0, 30°C, with 2,3-dihydroxybenzoate Coleus scutellarioides
0.0043
-
 4-coumaroyl-CoA pH 7.0, 30°C, with shikimate Coleus scutellarioides
0.0051
-
 caffeoyl-CoA pH 7.0, 30°C, with 3-hydroxybenzoate Coleus scutellarioides
0.0051
-
 feruloyl-CoA pH 7.0, 30°C, with shikimate Coleus scutellarioides
0.0088
-
 caffeoyl-CoA pH 7.0, 30°C, with shikimate Coleus scutellarioides
0.0118
-
 caffeoyl-CoA pH 7.0, 30°C, with 2,3-dihydroxybenzoate Coleus scutellarioides
0.0185
-
 4-coumaroyl-CoA pH 7.0, 30°C, with 3-aminobenzoate Coleus scutellarioides
0.02
-
 sinapoyl-CoA pH 7.0, 30°C, with shikimate Coleus scutellarioides
0.022
-
 4-coumaroyl-CoA pH 7.0, 30°C, with 3-hydroxyanthranilate Coleus scutellarioides
0.0265
-
 cinnamoyl-CoA pH 7.0, 30°C, with shikimate Coleus scutellarioides
0.0538
-
 caffeoyl-CoA pH 7.0, 30°C, with 3-hydroxyanthranilate Coleus scutellarioides
0.188
-
 2,3-Dihydroxybenzoate pH 7.0, 30°C, with 4-coumaroyl-CoA Coleus scutellarioides
0.332
-
 shikimate pH 7.0, 30°C, with 4-coumaroyl-CoA Coleus scutellarioides
0.449
-
 3-Hydroxyanthranilate pH 7.0, 30°C, with caffeoyl-CoA Coleus scutellarioides
0.473
-
 3-Hydroxyanthranilate pH 7.0, 30°C, with 4-coumaroyl-CoA Coleus scutellarioides
0.699
-
 2,3-Dihydroxybenzoate pH 7.0, 30°C, with caffeoyl-CoA Coleus scutellarioides
0.733
-
 3-hydroxybenzoate pH 7.0, 30°C, with caffeoyl-CoA Coleus scutellarioides
1.677
-
 3-hydroxybenzoate pH 7.0, 30°C, with 4-coumaroyl-CoA Coleus scutellarioides
2.235
-
 shikimate pH 7.0, 30°C, with cinnamoyl-CoA Coleus scutellarioides
2.404
-
 3-aminobenzoate pH 7.0, 30°C, with 4-coumaroyl-CoA Coleus scutellarioides
6.495
-
 shikimate pH 7.0, 30°C, with caffeoyl-CoA Coleus scutellarioides
22.38
-
 shikimate pH 7.0, 30°C, with feroyl-CoA Coleus scutellarioides
26.13
-
 shikimate pH 7.0, 30°C, with sinapoyl-CoA Coleus scutellarioides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47100
-
 x * 47100, about, sequence calculation Coleus scutellarioides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-coumaroyl-CoA + shikimate Coleus scutellarioides
-
 CoA + 5-O-(4-coumaroyl)shikimate
-
 ?

Organism

Organism UniProt Comment Textmining
Coleus scutellarioides E8ZAP2 gene cbhct2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged HST from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and dialysis Coleus scutellarioides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + 2,3-dihydroxybenzoate
-
 Coleus scutellarioides CoA + ?
-
 ?
4-coumaroyl-CoA + 3-aminobenzoate
-
 Coleus scutellarioides CoA + ?
-
 ?
4-coumaroyl-CoA + 3-hydroxyanthranilate
-
 Coleus scutellarioides CoA + 3-(4-coumaroyl)oxyanthranilate
-
 ?
4-coumaroyl-CoA + 3-hydroxybenzoate
-
 Coleus scutellarioides CoA + 3-(4-coumaroyl)oxybenzoate
-
 ?
4-coumaroyl-CoA + shikimate
-
 Coleus scutellarioides CoA + 5-O-(4-coumaroyl)shikimate
-
 ?
4-coumaroyl-CoA + shikimate preferred substrate for HST with shikimate as acceptor substrate is 4-coumaroyl-CoA Coleus scutellarioides CoA + 5-O-(4-coumaroyl)shikimate
-
 ?
caffeoyl-CoA + 2,3-dihydroxybenzoate
-
 Coleus scutellarioides CoA + ?
-
 ?
caffeoyl-CoA + 3-aminobenzoate
-
 Coleus scutellarioides CoA + ?
-
 ?
caffeoyl-CoA + 3-hydroxyanthranilate
-
 Coleus scutellarioides CoA + 3-caffeoyloxyanthranilate
-
 ?
caffeoyl-CoA + 3-hydroxybenzoate
-
 Coleus scutellarioides CoA + 3-caffeoyloxybenzoate
-
 ?
caffeoyl-CoA + shikimate
-
 Coleus scutellarioides CoA + 5-O-caffeoylshikimate
-
 r
cinnamoyl-CoA + 2,3-dihydroxybenzoate
-
 Coleus scutellarioides CoA + ?
-
 ?
cinnamoyl-CoA + 3-aminobenzoate
-
 Coleus scutellarioides CoA + ?
-
 ?
cinnamoyl-CoA + 3-hydroxyanthranilate
-
 Coleus scutellarioides CoA + 3-cinnamoyloxyanthranilate
-
 ?
cinnamoyl-CoA + 3-hydroxybenzoate
-
 Coleus scutellarioides CoA + 3-cinnamoyloxybenzoate
-
 ?
cinnamoyl-CoA + shikimate very low activity Coleus scutellarioides CoA + 5-O-cinnamoylshikimate
-
 r
feruloyl-CoA + 2,3-dihydroxybenzoate
-
 Coleus scutellarioides CoA + ?
-
 ?
feruloyl-CoA + 3-aminobenzoate
-
 Coleus scutellarioides CoA + ?
-
 ?
feruloyl-CoA + 3-hydroxyanthranilate
-
 Coleus scutellarioides CoA + 3-feruloyloxyanthranilate
-
 ?
feruloyl-CoA + 3-hydroxybenzoate
-
 Coleus scutellarioides CoA + 3-feruloyloxybenzoate
-
 ?
feruloyl-CoA + shikimate
-
 Coleus scutellarioides CoA + 5-O-feruloylshikimate
-
 r
additional information substrate specificity of recombinant HST: no activity with quinate and anthranilate, and tyramine, tryptamine, serotonin, dopamine, DOPA, 2-phenethylamine, phenol, as acceptors Coleus scutellarioides ?
-
 ?
sinapoyl-CoA + 3-hydroxyanthranilate
-
 Coleus scutellarioides CoA + 3-sinapoyloxyanthranilate
-
 r
sinapoyl-CoA + shikimate very low activity Coleus scutellarioides CoA + 5-O-sinapoylshikimate
-
 r

Subunits

Subunits Comment Organism
? x * 47100, about, sequence calculation Coleus scutellarioides

Synonyms

Synonyms Comment Organism
HST
-
 Coleus scutellarioides
hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyl-transferase
-
 Coleus scutellarioides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Coleus scutellarioides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Coleus scutellarioides

pI Value

Organism Comment pI Value Maximum pI Value
Coleus scutellarioides sequence calculation
-
 5.78

General Information

General Information Comment Organism
evolution HST belongs to the large plant acyl-CoA-dependent BAHD acyltransferase superfamily characterized by mostly conserved sequence motifs, the HxxxD(G) motif containing the catalytically active histidine residue and the DFGWG motif close to the C terminus, phylogenetic analysis, overview Coleus scutellarioides