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Literature summary for 2.3.1.150 extracted from
- Molecular characterization of the salutaridinol 7-O-acetyltransferase involved in morphine biosynthesis in opium poppy Papaver somniferum (2001), J. Biol. Chem., 276, 30717-30723.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | potential application in biotechnological production of morphine alkaloids | Papaver somniferum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as His-tag fusion protein in Sf9 insect cells | Papaver somniferum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.009 | - |
salutaridinol | - |
Papaver somniferum |  |
| 0.023 | - |
nudaurine | - |
Papaver somniferum | |
| 0.054 | - |
acetyl-CoA | with salutaridinol as substrate | Papaver somniferum | |
| 0.106 | - |
acetyl-CoA | with nudaurine as substrate | Papaver somniferum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52600 | - |
x * 52600, calculated from the deduced amino acid sequence | Papaver somniferum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + salutaridinol | Papaver somniferum | involved in morphine biosynthesis | CoA + 7-O-acetylsalutaridinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Papaver somniferum | Q94FT4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification from host organism and recombinant protein using His-tag | Papaver somniferum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + nudaurine | - |
Papaver somniferum | CoA + O-acetylnudaurine | - |
? | |
| acetyl-CoA + salutaridinol | - |
Papaver somniferum | CoA + 7-O-acetylsalutaridinol | - |
? | |
| acetyl-CoA + salutaridinol | involved in morphine biosynthesis | Papaver somniferum | CoA + 7-O-acetylsalutaridinol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52600, calculated from the deduced amino acid sequence | Papaver somniferum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 47 | - |
- |
Papaver somniferum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.3 | - |
nudaurine | - |
Papaver somniferum | |
| 4.4 | - |
salutaridinol | - |
Papaver somniferum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Papaver somniferum |
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Release 2023.1 (January 2023)
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