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Literature summary for 2.3.1.157 extracted from

  • Zhang, W.; Jones, V.C.; Scherman, M.S.; Mahapatra, S.; Crick, D.; Bhamidi, S.; Xin, Y.; McNeil, M.R.; Ma, Y.
    Expression, essentiality, and a microtiter plate assay for mycobacterial GlmU, the bifunctional glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridyltransferase (2008), Int. J. Biochem. Cell Biol., 40, 2560-2571.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.157

Application

Application Comment Organism
medicine GlmU is a potential antituberculosis drug target, a microtiter plate assay for GlmU activity as read out is developed Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, expression in Mycobacterium smegmatis ZW-2, a GlmU knoukout strain, activity of GlmU is required for growth of Mycobacterium smegmatis, a gradually reduced enzyme activity in a temperature shift experiment shows that the Mycobacterium smegmatis cells became non-viable and their morphology changes from a normal rod shape to stubby-rounded morphology Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54100
-
 SDS-PAGE, Western blot Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WMN3
-
-
Mycobacterium tuberculosis H37Rv P9WMN3
-
-

Purification (Commentary)

Purification (Comment) Organism
the recombinant protein by Ni2+ affinity chromatography Mycobacterium tuberculosis

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate also N-acetyl-alpha-D-glucosamine 1-phosphate + UTP = UDP-N-acetyl-D-glucosamine + diphosphate Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Mycobacterium tuberculosis CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Mycobacterium tuberculosis H37Rv CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
UDP-N-acetyl-d-glucosamine + diphosphate
-
 Mycobacterium tuberculosis N-acetyl-alpha-D-glucosamine 1-phosphate + UTP
-
 r
UDP-N-acetyl-d-glucosamine + diphosphate
-
 Mycobacterium tuberculosis H37Rv N-acetyl-alpha-D-glucosamine 1-phosphate + UTP
-
 r

Synonyms

Synonyms Comment Organism
GlmU
-
 Mycobacterium tuberculosis
glucosamine-1-phosphate acetyltransferase bifuncional enzyme, also N-acetylglucosamine-1-phosphate uridyltransferase activity Mycobacterium tuberculosis