Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | MtGlmU has acetyltransferase activity in the absence of reducing agent and in the presence of a thiolreactive reagent (N-ethylmaleimide) | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
the crystal structures of MtGlmU in an unliganded form and in complexes in which either GlcNAc-1-P or UDP-GlcNAc occupies the uridyltransferase active site are determined using the sitting-drop vapour-diffusion method. These structures identify the active-site contacts between protein and ligands and suggest a ternary-complex mechanism of action for the GlmU uridyltransferase reaction | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WMN3 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WMN3 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography. The His6 tag is cleaved by incubation with recombinant tobacco etch virus (rTEV) protease | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucosamine 1-phosphate + acetyl-CoA | presence of acetyl-coenzyme A has an inhibitory effect on uridyltransferase activity of the bifunctional enzyme | Mycobacterium tuberculosis | N-acetyl-D-glucosamine 1-phosphate + CoA | - |
? | |
D-glucosamine 1-phosphate + acetyl-CoA | presence of acetyl-coenzyme A has an inhibitory effect on uridyltransferase activity of the bifunctional enzyme | Mycobacterium tuberculosis H37Rv | N-acetyl-D-glucosamine 1-phosphate + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MtGlmU | bifunctional enzyme also possesses the activity of EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase | Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycobacterium tuberculosis |