Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.157 extracted from

  • Zhang, Z.; Akutsu, J.; Kawarabayasi, Y.
    Identification of novel acetyltransferase activity on the thermostable protein ST0452 from Sulfolobus tokodaii strain 7 (2010), J. Bacteriol., 192, 3287-3293.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.157

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 2 mM, inhibits D-glucosamine-1-phosphate N-acetyltransferase activity Sulfurisphaera tokodaii
Co2+ 2 mM, inhibits D-glucosamine-1-phosphate N-acetyltransferase activity Sulfurisphaera tokodaii
Mg2+ 2 mM, inhibits D-glucosamine-1-phosphate N-acetyltransferase activity Sulfurisphaera tokodaii
Mn2+ 2 mM, inhibits D-glucosamine-1-phosphate N-acetyltransferase activity Sulfurisphaera tokodaii
Zn2+ 2 mM, inhibits D-glucosamine-1-phosphate N-acetyltransferase activity Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.59
-
 alpha-D-glucosamine 1-phosphate pH 7.5, 80°C Sulfurisphaera tokodaii
0.63
-
 acetyl-CoA pH 7.5, 80°C Sulfurisphaera tokodaii

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ion required Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + alpha-D-glucosamine 1-phosphate Sulfurisphaera tokodaii because the ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
acetyl-CoA + alpha-D-glucosamine 1-phosphate Sulfurisphaera tokodaii 7 because the ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q975F9
-
-
Sulfurisphaera tokodaii 7 Q975F9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfurisphaera tokodaii

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate the multifunctional enzyme from Sulfolobus tokodaii {7} is also active with acetyl-CoA + alpha-D-galactosamine 1-phosphate (galactosamine-1-phosphate N-acetyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase) and UTP + N-acetyl-alpha-D-galactosamine 1-phosphate (EC 2.7.7.83, UDP-N-acetylgalactosamine diphosphorylase) Sulfurisphaera tokodaii
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
75.34
-
 pH 7.5, 80°C, substrates: acetyl-CoA + alpha-D-glucosamine 1-phosphate Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + alpha-D-glucosamine 1-phosphate because the ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon Sulfurisphaera tokodaii CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
acetyl-CoA + alpha-D-glucosamine 1-phosphate no activity with alpha-D-glucosamine 6-phosphate. The multifunctional enzyme is also active with acetyl-CoA + alpha-D-galactosamine 1-phosphate (galactosamine-1-phosphate N-acetyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase) and UTP + N-acetyl-alpha-D-galactosamine 1-phosphate (EC 2.7.7.83, UDP-N-acetylgalactosamine diphosphorylase) Sulfurisphaera tokodaii CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
acetyl-CoA + alpha-D-glucosamine 1-phosphate because the ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon Sulfurisphaera tokodaii 7 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
acetyl-CoA + alpha-D-glucosamine 1-phosphate no activity with alpha-D-glucosamine 6-phosphate. The multifunctional enzyme is also active with acetyl-CoA + alpha-D-galactosamine 1-phosphate (galactosamine-1-phosphate N-acetyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase) and UTP + N-acetyl-alpha-D-galactosamine 1-phosphate (EC 2.7.7.83, UDP-N-acetylgalactosamine diphosphorylase) Sulfurisphaera tokodaii 7 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 assay at Sulfurisphaera tokodaii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
123.2
-
 alpha-D-glucosamine 1-phosphate pH 7.5, 80°C Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Sulfurisphaera tokodaii

General Information

General Information Comment Organism
physiological function because the ST0452 protein is capable of catalyzing the last two reactions (Ec 2.3.1.157 and EC 2.7.7.23 (UDP-N-acetylglucosamine diphosphorylase)) of the bacteria-type four-step biosynthesis pathway of UDP-GlcNAc from fructose 6-phosphate, the ST0452 protein plays an important role for the bacteria-type UDP-GlcNAc biosynthesis pathway in this archaeon Sulfurisphaera tokodaii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
211
-
 alpha-D-glucosamine 1-phosphate pH 7.5, 80°C Sulfurisphaera tokodaii