Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Citrus x microcarpa |
Crystallization (Comment) | Organism |
---|---|
to 2.35 A resolution. Structure reveals wide active site entrances providing sufficient space to facilitate the binding of the bulky N-methylanthraniloyl-CoA within the catalytic center | Citrus x microcarpa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0043 | - |
N-methylanthraniloyl-CoA | product 1,3-dihydroxy-N-methylacridone, pH 7.0, 30°C | Citrus x microcarpa | |
0.0136 | - |
4-coumaroyl-CoA | pH 7.0, 30°C | Citrus x microcarpa | |
0.0374 | - |
N-methylanthraniloyl-CoA | product 4-hydroxy-N-methylquinolone, pH 7.0, 30°C | Citrus x microcarpa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus x microcarpa | U3KRF0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 malonyl-CoA + 4-coumaroyl-CoA | - |
Citrus x microcarpa | 4 CoA + naringenin chalcone + 3 CO2 | enzyme accepts 4-coumaroyl-CoA as a starter substrate to yield naringenin chalcone, along with triketide and tetraketide lactone derailment by-products | ? | |
3 malonyl-CoA + benzoyl-CoA | - |
Citrus x microcarpa | 4 CoA + benzophenone + 3 CO2 | - |
? | |
3 malonyl-CoA + hexanoyl-CoA | - |
Citrus x microcarpa | 4 CoA + phloroglucinol + 3 CO2 | - |
? | |
3 malonyl-CoA + N-methylanthraniloyl-CoA | - |
Citrus x microcarpa | 4 CoA + 1,3-dihydroxy-N-methylacridone + N-methylanthraniloyltriacetic acid lactone + 4-hydroxy-N-methylquinolone + 3 CO2 | enzyme accepts N-methylanthraniloyl-CoA as the starter substrate to produce the tetraketide 1,3-dihydroxy-N-methylacridone after sequential condensations with three molecules of malonyl-CoA, along with 4-hydroxy-N-methylquinolone and N-methylanthraniloyltriacetic acid lactone. The catalytic efficiency for the formation of the acridone is 2.8fold higher than that of the quinolone | ? |
Synonyms | Comment | Organism |
---|---|---|
ACS | - |
Citrus x microcarpa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
N-methylanthraniloyl-CoA | product 1,3-dihydroxy-N-methylacridone, pH 7.0, 30°C | Citrus x microcarpa | |
0.067 | - |
N-methylanthraniloyl-CoA | product 4-hydroxy-N-methylquinolone, pH 7.0, 30°C | Citrus x microcarpa | |
0.113 | - |
4-coumaroyl-CoA | pH 7.0, 30°C | Citrus x microcarpa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.95 | - |
N-methylanthraniloyl-CoA | product 4-hydroxy-N-methylquinolone, pH 7.0, 30°C | Citrus x microcarpa | |
5.4 | - |
N-methylanthraniloyl-CoA | product 1,3-dihydroxy-N-methylacridone, pH 7.0, 30°C | Citrus x microcarpa | |
8.33 | - |
4-coumaroyl-CoA | pH 7.0, 30°C | Citrus x microcarpa |