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Literature summary for 2.3.1.159 extracted from

  • Mori, T.; Shimokawa, Y.; Matsui, T.; Kinjo, K.; Kato, R.; Noguchi, H.; Sugio, S.; Morita, H.; Abe, I.
    Cloning and structure-function analyses of quinolone- and acridone-producing novel type III polyketide synthases from Citrus microcarpa (2013), J. Biol. Chem., 288, 28845-28858.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Citrus x microcarpa

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.35 A resolution. Structure reveals wide active site entrances providing sufficient space to facilitate the binding of the bulky N-methylanthraniloyl-CoA within the catalytic center Citrus x microcarpa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0043
-
N-methylanthraniloyl-CoA product 1,3-dihydroxy-N-methylacridone, pH 7.0, 30°C Citrus x microcarpa
0.0136
-
4-coumaroyl-CoA pH 7.0, 30°C Citrus x microcarpa
0.0374
-
N-methylanthraniloyl-CoA product 4-hydroxy-N-methylquinolone, pH 7.0, 30°C Citrus x microcarpa

Organism

Organism UniProt Comment Textmining
Citrus x microcarpa U3KRF0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 malonyl-CoA + 4-coumaroyl-CoA
-
Citrus x microcarpa 4 CoA + naringenin chalcone + 3 CO2 enzyme accepts 4-coumaroyl-CoA as a starter substrate to yield naringenin chalcone, along with triketide and tetraketide lactone derailment by-products ?
3 malonyl-CoA + benzoyl-CoA
-
Citrus x microcarpa 4 CoA + benzophenone + 3 CO2
-
?
3 malonyl-CoA + hexanoyl-CoA
-
Citrus x microcarpa 4 CoA + phloroglucinol + 3 CO2
-
?
3 malonyl-CoA + N-methylanthraniloyl-CoA
-
Citrus x microcarpa 4 CoA + 1,3-dihydroxy-N-methylacridone + N-methylanthraniloyltriacetic acid lactone + 4-hydroxy-N-methylquinolone + 3 CO2 enzyme accepts N-methylanthraniloyl-CoA as the starter substrate to produce the tetraketide 1,3-dihydroxy-N-methylacridone after sequential condensations with three molecules of malonyl-CoA, along with 4-hydroxy-N-methylquinolone and N-methylanthraniloyltriacetic acid lactone. The catalytic efficiency for the formation of the acridone is 2.8fold higher than that of the quinolone ?

Synonyms

Synonyms Comment Organism
ACS
-
Citrus x microcarpa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.023
-
N-methylanthraniloyl-CoA product 1,3-dihydroxy-N-methylacridone, pH 7.0, 30°C Citrus x microcarpa
0.067
-
N-methylanthraniloyl-CoA product 4-hydroxy-N-methylquinolone, pH 7.0, 30°C Citrus x microcarpa
0.113
-
4-coumaroyl-CoA pH 7.0, 30°C Citrus x microcarpa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.95
-
N-methylanthraniloyl-CoA product 4-hydroxy-N-methylquinolone, pH 7.0, 30°C Citrus x microcarpa
5.4
-
N-methylanthraniloyl-CoA product 1,3-dihydroxy-N-methylacridone, pH 7.0, 30°C Citrus x microcarpa
8.33
-
4-coumaroyl-CoA pH 7.0, 30°C Citrus x microcarpa