Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | di-(2-ethylhexyl)phthalate induces the enzyme | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetoacetyl-CoA | competitive inhibition of the acetyl-CoA hydrolase activity | Rattus norvegicus | |
acetyl-CoA | competitive inhibition of the 3-ketoacyl-CoA thiolase activity | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Rattus norvegicus | |
0.0114 | - |
acetoacetyl-CoA | pH 7.4 | Rattus norvegicus | |
0.71 | - |
acetyl-CoA | pH 7.4 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O | Rattus norvegicus | 3-ketoacyl-CoA thiolase activity | acetate + CoA | - |
? | |
additional information | Rattus norvegicus | the enzyme is involved in fatty acid beta-oxidation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 27.2fold from liver mitochondria preparation by 5 steps of ion exchange and hydrophobic interaction chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brown adipose tissue | di-(2-ethylhexyl)phthalate-inducible acetyl-CoA hydrolase activity | Rattus norvegicus | - |
heart | di-(2-ethylhexyl)phthalate-inducible acetyl-CoA hydrolase activity | Rattus norvegicus | - |
intestine | di-(2-ethylhexyl)phthalate-inducible acetyl-CoA hydrolase activity | Rattus norvegicus | - |
kidney | di-(2-ethylhexyl)phthalate-inducible acetyl-CoA hydrolase activity | Rattus norvegicus | - |
liver | di-(2-ethylhexyl)phthalate-inducible acetyl-CoA hydrolase activity | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.543 | - |
purified enzyme, acetyl-CoA hydrolase activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O | 3-ketoacyl-CoA thiolase activity | Rattus norvegicus | acetate + CoA | - |
? | |
acetyl-CoA + H2O | best acyl-CoA substrate, acetyl-CoA hydrolase activity | Rattus norvegicus | acetate + CoA | - |
? | |
acetyl-CoA + H2O | preferred substrate, 3-ketoacyl-CoA thiolase activity | Rattus norvegicus | acetate + CoA | - |
? | |
butyryl-CoA + H2O | acetyl-CoA hydrolase activity | Rattus norvegicus | butanoate + CoA | - |
? | |
dodecanoyl-CoA + H2O | acetyl-CoA hydrolase activity | Rattus norvegicus | dodecanoate + CoA | - |
? | |
hexanoyl-CoA + H2O | acetyl-CoA hydrolase activity | Rattus norvegicus | hexanoate + CoA | - |
? | |
additional information | the enzyme is involved in fatty acid beta-oxidation | Rattus norvegicus | ? | - |
? | |
additional information | analysis of the substrate chain length specificity of the enzyme, high preference for acetyl-CoA | Rattus norvegicus | ? | - |
? | |
palmitoyl-CoA + H2O | acetyl-CoA hydrolase activity | Rattus norvegicus | palmitate + CoA | - |
? | |
propionyl-CoA + H2O | acetyl-CoA hydrolase activity | Rattus norvegicus | propionate + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-ketoacyl-CoA thiolase | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
acetoacetyl-CoA | pH 7.4, competitive versus acetyl-CoA hydrolysis | Rattus norvegicus | |
0.24 | - |
acetyl-CoA | pH 7.4, competitive versus 3-ketoacyl-CoA thiolase activity | Rattus norvegicus |