Literature summary for 2.3.1.161 extracted from

Auclair, K.; Sutherland, A.; Kennedy, J.; Witter, D.J.; Van den Heever, J.P.; Hutchinson, C.R.; Vederas, J.C.
Lovastatin nonaketide synthase catalyzes an intramolecular Diels-Alder reaction of a substrate analogue (2000), J. Am. Chem. Soc., 122, 11519-11520.

No PubMed abstract available

Search for more literature for 2.3.1.161

Application

Application	Comment	Organism
medicine	production of cholesterol lowering drugs	Aspergillus terreus

Cloned(Commentary)

Cloned (Comment)	Organism
lovB and lovC protein	Aspergillus terreus

General Stability

General Stability	Organism
inactivated by heating	Aspergillus terreus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.5	-	malonyl-CoA	-	Aspergillus terreus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
335000	-	SDS-PAGE	Aspergillus terreus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetyl-CoA + 8 malonyl-CoA + 11 NADPH + S-adenosyl-L-methionine + 11 H+	Aspergillus terreus	reaction mechanism	dihydromonacolin L + 9 CoA + 8 CO2 + 11 NADP+ + S-adenosyl-L-homocysteine + 6 H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Aspergillus terreus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
homogeneitiy	Aspergillus terreus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0109	-	-	Aspergillus terreus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + 8 malonyl-CoA + 11 NADPH + S-adenosyl-L-methionine + 11 H+	reaction mechanism	Aspergillus terreus	dihydromonacolin L + 9 CoA + 8 CO2 + 11 NADP+ + S-adenosyl-L-homocysteine + 6 H2O	-	?
acetyl-CoA + 8 malonyl-CoA + 11 NADPH + S-adenosyl-L-methionine + 11 H+	enzyme also displays Diels-Alderase activity in vitro	Aspergillus terreus	dihydromonacolin L + 9 CoA + 8 CO2 + 11 NADP+ + S-adenosyl-L-homocysteine + 6 H2O	-	?

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Release 2023.1 (January 2023)