General Stability | Organism |
---|---|
PMSF and benzamidine in disruption buffer extend half-life from 20 min to 6 h, 15% glycerol and proteinase inhibitors further improve stability | Penicillium griseofulvum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,3-dibromo-propane-2-one | 0.02 mM, complete inactivation after 40 min, acetyl-CoA protects | Penicillium griseofulvum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
malonyl-CoA | - |
Penicillium griseofulvum | |
0.01 | - |
acetyl-CoA | - |
Penicillium griseofulvum | |
0.012 | - |
NADPH | - |
Penicillium griseofulvum | |
0.065 | - |
acetoacetyl-CoA | - |
Penicillium griseofulvum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | - |
alpha4, 4 * 180000, SDS-PAGE | Penicillium griseofulvum |
750000 | - |
gel filtration | Penicillium griseofulvum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium griseofulvum | - |
NRRL 2159A | - |
Penicillium griseofulvum | - |
also known as Penicillium urticae | - |
Penicillium griseofulvum NRRL 2159A | - |
NRRL 2159A | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, poly(ethylene glycol) 6000, DEAE-Sepharose, hydroxyapatite, Mono Q | Penicillium griseofulvum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + 3 malonyl-CoA + NADPH + H+ = 6-methylsalicylate + 4 CoA + 3 CO2 + NADP+ + H2O | a multienzyme complex with a 4'-phosphopantetheine prosthetic group on the acyl carrier protein, it has a similar sequence to vertebrate type I fatty acid synthase, acetoacetyl-CoA can also act as a starter molecule | Penicillium griseofulvum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.245 | - |
- |
Penicillium griseofulvum |
Storage Stability | Organism |
---|---|
-70°C, ammonium sulfate precipitate, loss of 5% activity per month | Penicillium griseofulvum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + malonyl-CoA + NADPH | - |
Penicillium griseofulvum | ? | - |
? | |
acetoacetyl-CoA + malonyl-CoA + NADPH | - |
Penicillium griseofulvum NRRL 2159A | ? | - |
? | |
acetyl-CoA + malonyl-CoA | exclusive product in the absence of NADPH, 5:1 ratio of 6-methylsalicylic acid to triacetic acid lactone at 0.0005 mM NADPH, less than 1% triacetic acid lactone at 0.012 mM NADPH | Penicillium griseofulvum | triacetic lactone | - |
? | |
acetyl-CoA + malonyl-CoA | exclusive product in the absence of NADPH, 5:1 ratio of 6-methylsalicylic acid to triacetic acid lactone at 0.0005 mM NADPH, less than 1% triacetic acid lactone at 0.012 mM NADPH | Penicillium griseofulvum NRRL 2159A | triacetic lactone | - |
? | |
acetyl-CoA + malonyl-CoA + NADPH | - |
Penicillium griseofulvum | 6-methylsalicylate + CoA + CO2 + NADP+ | - |
? | |
acetyl-CoA + malonyl-CoA + NADPH | - |
Penicillium griseofulvum NRRL 2159A | 6-methylsalicylate + CoA + CO2 + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha4, 4 * 180000, SDS-PAGE | Penicillium griseofulvum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Penicillium griseofulvum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | - |
Penicillium griseofulvum |