Cloned (Comment) | Organism |
---|---|
expression of apo-enzyme in Escherichia coli strain XL1-Blue, can be lipoylated in vitro | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant apo-enzyme E2 from Escherichia coli strain XL1-Blue | Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine = CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine | the active site is located in the inner core domain | Bos taurus |
Renatured (Comment) | Organism |
---|---|
recombinant apo-E2 is unable to reconstitute with recombinant E1 and E3 to an active branched-chain alpha-keto dehydrogenase, but recombinant holo-E2 is able to | Bos taurus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.173 | - |
purified recombinant apo-enzyme | Bos taurus |
0.185 | - |
purified native enzyme | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylpropanoyl-CoA + enzyme N6-(dihydrolipoyl)lysine | recombinant apo-E2 devoid of lipoic acid is fully active | Bos taurus | CoA + enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | recombinant apo-E2 is unable to reconstitute with recombinant E1 and E3 to an active branched-chain alpha-keto dehydrogenase, but recombinant holo-E2 is able to | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme is the E2 component of the multienzyme complex branched-chain alpha-keto acid dehydrogenase, i.e. branched-chain 2-oxo acid dehydrogenase | Bos taurus |