Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.169 extracted from

  • Roberts, D.L.; James-Hagstrom, J.E.; Garvin, D.K.; Gorst, C.M.; Runquist, J.A.; Baur, J.R.; Haase, F.C.; Ragsdale, S.W.
    Cloning and expression of the gene cluster encoding key proteins involved in acetyl-CoA synthesis in Clostridium thermoaceticum: carbon monoxide dehydrogenase, the corrinoid/iron-sulfur protein, and methyltransferase (1989), Proc. Natl. Acad. Sci. USA, 86, 32-36.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.169

Cloned(Commentary)

Cloned (Comment) Organism
the gene has been cloned into Escherichia coli and found to be within an 11 kb gene cluster, recombinant enzyme is inactive Moorella thermoacetica

Metals/Ions

Metals/Ions Comment Organism Structure
Fe corrinoid/iron-sulfur protein required Moorella thermoacetica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
-
 Moorella thermoacetica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Moorella thermoacetica key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway ?
-
 ?

Organism

Organism UniProt Comment Textmining
Moorella thermoacetica
-
 DSM 521
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway Moorella thermoacetica ?
-
 ?

Subunits

Subunits Comment Organism
dimer alpha,beta Moorella thermoacetica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
67
-
 the recombinant enzyme: 10 min, 10% loss of activity, the wild-type enzyme: 10 min, no loss of activity Moorella thermoacetica
75
-
 the wild-type enzyme: 10 min, 50% loss of activity Moorella thermoacetica