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Literature summary for 2.3.1.17 extracted from

  • Madhavarao, C.N.; Chinopoulos, C.; Chandrasekaran, K.; Namboodiri, M.A.
    Characterization of the N-acetylaspartate biosynthetic enzyme from rat brain (2003), J. Neurochem., 86, 824-835.
    View publication on PubMed
Search for more literature for 2.3.1.17

Inhibitors

Inhibitors Comment Organism Structure
CHAPS 40% of activity at 12 mM, 10% of activity at 20 mM Rattus norvegicus
CoA IC50: 0.42 mM Rattus norvegicus
N-acetyl-L-aspartic acid IC50: 0.85 mM Rattus norvegicus
NaCl above 150 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.058
-
 acetyl-CoA pH 7.1, 37°C Rattus norvegicus
0.58
-
 L-aspartate pH 7.1, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
670000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-aspartate Rattus norvegicus decreases in enzyme activity occur in a number of neurological disorders, possible role in neuronal mitochondrial energy metabolism CoA + N-acetyl-L-aspartic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 male sprague-dawley rats
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
neuron
-
 Rattus norvegicus
-
oligodendrocyte cultured Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-aspartate
-
 Rattus norvegicus CoA + N-acetyl-L-aspartic acid
-
 ?
acetyl-CoA + L-aspartate decreases in enzyme activity occur in a number of neurological disorders, possible role in neuronal mitochondrial energy metabolism Rattus norvegicus CoA + N-acetyl-L-aspartic acid
-
 ?
additional information highly specific for L-aspartate with 3% or less active for L-glutamate, L-asparagine, L-glutamine, or aspartate-glutamate dipeptide Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
multimer at least 10 bands on SDS-PAGE indicating a large enzyme complex Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.42
-
 IC50: 0.42 mM Rattus norvegicus CoA
0.85
-
 IC50: 0.85 mM Rattus norvegicus N-acetyl-L-aspartic acid