Literature summary for 2.3.1.17 extracted from

Wiame, E.; Tyteca, D.; Pierrot, N.; Collard, F.; Amyere, M.; Noel, G.; Desmedt, J.; Nassogne, M.C.; Vikkula, M.; Octave, J.N.; Vincent, M.F.; Courtoy, P.J.; Boltshauser, E.; van Schaftingen, E.
Molecular identification of aspartate N-acetyltransferase and its mutation in hypoacetylaspartia (2010), Biochem. J., 425, 127-136.

Search for more literature for 2.3.1.17

Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed both as native and as fusion protein with a His6 tag at the C- or the N-terminus in human embryonic kidney-293 cells expressing the large T-antigen of simian virus 40 (HEK-293T cells)	Homo sapiens
overexpressed both as native and as fusion proteins with a His6 tag at the C- or the N-terminus in human embryonic kidney-293 cells expressing the large T-antigen of simian virus 40 (HEK-293T cells); furthermore expressed as fusion protein in rat embryonal cortical neurons and in Chinese-hamster ovary cells	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
glutamate	Glutamate is a competitive inhibitor. No inhibition is observed with other amino acids, indicating that the enzyme is specific.	Mus musculus
N-acetyl-L-aspartate	-	Mus musculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.009	-	acetyl-CoA	in presence of 0.5 mM CHAPS	Mus musculus
0.013	-	acetyl-CoA	in presence of 1 mM CHAPS	Mus musculus
0.09	-	L-aspartate	-	Mus musculus
5	-	glutamate	-	Mus musculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	-	Mus musculus	5783	-
additional information	not in mitochondria	Mus musculus	-	-

Organism

Organism	UniProt	Comment	Textmining
Danio rerio	-	-	-
Homo sapiens	Q8N9F0	-	-
Mus musculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Mus musculus
-	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Mus musculus	-
brain	-	Homo sapiens	-
additional information	In PCR, no specific amplification was observed with liver, kidney, lung, skeletal muscle, testis and heart cDNA of mice.	Mus musculus	-
spleen	-	Mus musculus	-
thymus	-	Mus musculus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0015	-	activity of cloned mouse NAT8L fusion protein with N-terminal His6 tag	Mus musculus
0.0018	-	activity of cloned mouse NAT8L fusion protein with C-terminal His6 tag	Mus musculus
0.0025	-	activity of cloned wild type mouse NAT8L protein	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + glutamate	[14C]glutamate is acetylated with an approximately 50-fold lower affinity and a similar Vmax compared with aspartate	Mus musculus	CoA + N-acetylglutamate	-	?
acetyl-CoA + L-aspartate	-	Mus musculus	CoA + N-acetyl-L-aspartic acid	-	?
acetyl-CoA + L-aspartate	-	Homo sapiens	CoA + N-acetyl-L-aspartic acid	-	?

Synonyms

Synonyms	Comment	Organism
aspartate N-acetyltransferase	-	Mus musculus
aspartate N-acetyltransferase	-	Danio rerio
aspartate N-acetyltransferase	-	Homo sapiens
NAT	-	Mus musculus
NAT	-	Danio rerio
NAT	-	Homo sapiens
NAT8L	-	Mus musculus
NAT8L	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.56	-	N-acetyl-L-aspartate	-	Mus musculus
6.1	-	glutamate	-	Mus musculus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)