Cloned (Comment) | Organism |
---|---|
expression in HEK-293T cell | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C128A | mutation in region 4, 94% of wild-type expression, 88% of wild-type activity | Homo sapiens |
C139A | mutation in region 4, 70% of wild-type expression, 126% of wild-type activity | Homo sapiens |
D168A | mutation in region 5, 46% of wild-type expression, no residual activity | Homo sapiens |
D168E | mutation in region 5, 63% of wild-type expression, 7% of wild-type activity | Homo sapiens |
E101A | mutation in region 3, 97% of wild-type expression, 42% of wild-type activity | Homo sapiens |
E101D | mutation in region 3, 61% of wild-type expression, 99% of wild-type activity | Homo sapiens |
additional information | transfection of truncated forms of isoform NAT8L into HEK-293T cells indicates that the 68 N-terminal residues, i.e. regions 1 and 2, have no importance for the catalytic activity and the subcellular localization of the enzyme. The membrane region, i.e. region 4, is necessary and sufficient to target isoform NAT8L to the endoplasmic reticulum | Homo sapiens |
P142A | mutation in region 4, 113% of wild-type expression, 116% of wild-type activity | Homo sapiens |
R133A | mutation in region 4, 78% of wild-type expression, 64% of wild-type activity | Homo sapiens |
R133K | mutation in region 4, 69% of wild-type expression, 89% of wild-type activity | Homo sapiens |
R220A | mutation in region 5, 101% of wild-type expression, 14% of wild-type activity | Homo sapiens |
R220K | mutation in region 5, 104% of wild-type expression, 25% of wild-type activity | Homo sapiens |
R81A | mutation in region 3, 57% of wild-type expression, 37% of wild-type activity | Homo sapiens |
R81K | mutation in region 3, 90% of wild-type expression, 82% of wild-type activity | Homo sapiens |
S132F/R133F | mutation in region 4, 92% of wild-type expression, 41% of wild-type activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
acetyl-CoA | wild-type, pH 7.1, 30°C | Homo sapiens | |
0.012 | - |
acetyl-CoA | mutant P142A, pH 7.1, 30°C | Homo sapiens | |
0.017 | - |
acetyl-CoA | mutant S132F/R133F, pH 7.1, 30°C | Homo sapiens | |
0.019 | - |
acetyl-CoA | mutant R133A, pH 7.1, 30°C | Homo sapiens | |
0.082 | - |
acetyl-CoA | mutant R81A, pH 7.1, 30°C | Homo sapiens | |
0.09 | - |
L-aspartate | wild-type, pH 7.1, 30°C | Homo sapiens | |
0.095 | - |
L-aspartate | mutant P142A, pH 7.1, 30°C | Homo sapiens | |
0.127 | - |
acetyl-CoA | mutant E101A, pH 7.1, 30°C | Homo sapiens | |
0.128 | - |
L-aspartate | mutant S132F/R133F, pH 7.1, 30°C | Homo sapiens | |
0.14 | - |
acetyl-CoA | mutant R220K, pH 7.1, 30°C | Homo sapiens | |
0.2 | - |
L-aspartate | mutant R81A, pH 7.1, 30°C | Homo sapiens | |
0.4 | - |
acetyl-CoA | mutant R220A, pH 7.1, 30°C | Homo sapiens | |
0.434 | - |
L-aspartate | mutant E101A, pH 7.1, 30°C | Homo sapiens | |
1.61 | - |
L-aspartate | mutant R220K, pH 7.1, 30°C | Homo sapiens | |
3.37 | - |
L-aspartate | mutant R220A, pH 7.1, 30°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | exclusively associated with the endoplasmic reticulum. the membrane region comprises alpha-helices and the catalytic site is in the cytosol. The membrane region, i.e. region 4, is necessary and sufficient to target isoform NAT8L to the endoplasmic reticulum | Homo sapiens | 5783 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8N9F0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-aspartate | - |
Homo sapiens | CoA + N-acetyl-L-aspartic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAT8L | - |
Homo sapiens |