Literature summary for 2.3.1.175 extracted from

Matsuyama, K.; Matsumoto, H.; Matsuda, A.; Sugiura, H.; Komatsu, K.; Ichikawa, S.
Purification of acetyl coenzyme A: deacetylacephalosporin C O-acetyltransferase from Acremonium chrysogenum (1992), Biosci. Biotechnol. Biochem., 56, 1410-1412.

Search for more literature for 2.3.1.175

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.29	-	deacetylcephalosporin C	-	Acremonium chrysogenum
1.05	-	acetyl-CoA	-	Acremonium chrysogenum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	gel filtration	Acremonium chrysogenum

Organism

Organism	UniProt	Comment	Textmining
Acremonium chrysogenum	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	DCPC-ATF is synthesized as a single-chain precursor protein and is then converted to the two constituent subunit DCPC-ATF by proteolytic processing	Acremonium chrysogenum

Purification (Commentary)

Purification (Comment)	Organism
-	Acremonium chrysogenum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.3	-	-	Acremonium chrysogenum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + deacetylcephalosporin C	-	Acremonium chrysogenum	CoA + cephalosporin C	-	?

Subunits

Subunits	Comment	Organism
?	SDS-PAGE resolves two protein bands with molecular weights of 27000 Da and 14000 Da	Acremonium chrysogenum

Synonyms

Synonyms	Comment	Organism
acetyl coenzyme A: deacetylacephalosporin C O-acetyltransferase	-	Acremonium chrysogenum
DCPC-ATF	-	Acremonium chrysogenum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Acremonium chrysogenum

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	8.5	pH 6.0: about 60% of maximal activity, pH 8.5: about 50% of maximal activity	Acremonium chrysogenum

pI Value

Organism	Comment	pI Value Maximum	pI Value
Acremonium chrysogenum	chromatofocusing	-	4

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Release 2023.1 (January 2023)