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Literature summary for 2.3.1.178 extracted from

  • Reshetnikov, A.S.; Mustakhimov, II; Khmelenina, V.N.; Trotsenko, Y.A.
    Cloning, purification, and characterization of diaminobutyrate acetyltransferase from the halotolerant methanotroph Methylomicrobium alcaliphilum 20Z (2005), Biochemistry (Moscow), 70, 878-883.
    View publication on PubMed
Search for more literature for 2.3.1.178

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli with an additional six His residues at the C-terminus Methylotuvimicrobium alcaliphilum

Inhibitors

Inhibitors Comment Organism Structure
ATP 10 mM, 26% inhibition, 1 mM, 18% inhibition Methylotuvimicrobium alcaliphilum
Cd2+ 1 mM, complete inhibition Methylotuvimicrobium alcaliphilum
Zn2+ 1 mM, complete inhibition Methylotuvimicrobium alcaliphilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0366
-
 acetyl-CoA
-
 Methylotuvimicrobium alcaliphilum
0.46
-
 L-2,4-diaminobutanoate
-
 Methylotuvimicrobium alcaliphilum

Metals/Ions

Metals/Ions Comment Organism Structure
KCl 0.25 M, activity increases threefold Methylotuvimicrobium alcaliphilum
NaCl 0.1-0.15 M, activity increases threefold Methylotuvimicrobium alcaliphilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 2 * 20000, SDS-PAGE Methylotuvimicrobium alcaliphilum
40000
-
 gel filtration Methylotuvimicrobium alcaliphilum

Organism

Organism UniProt Comment Textmining
Methylotuvimicrobium alcaliphilum
-
 20Z
-
Methylotuvimicrobium alcaliphilum 20Z
-
 20Z
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methylotuvimicrobium alcaliphilum

Storage Stability

Storage Stability Organism
4°C or -70°C, protein concentration 0.5 mg/ml, stable for at least 1 month in 50 mM Tris-HCl, pH 8.5, containing 0.2 M KCl Methylotuvimicrobium alcaliphilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-2,4-diaminobutanoate
-
 Methylotuvimicrobium alcaliphilum CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
acetyl-CoA + L-2,4-diaminobutanoate
-
 Methylotuvimicrobium alcaliphilum 20Z CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
propionyl-CoA + L-2,4-diaminobutanoate 20% of the activity with acetyl-CoA Methylotuvimicrobium alcaliphilum CoA + N4-propionyl-L-2,4-diaminobutanoate
-
 ?
propionyl-CoA + L-2,4-diaminobutanoate 20% of the activity with acetyl-CoA Methylotuvimicrobium alcaliphilum 20Z CoA + N4-propionyl-L-2,4-diaminobutanoate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 20000, SDS-PAGE Methylotuvimicrobium alcaliphilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
 Methylotuvimicrobium alcaliphilum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 25 15°C: about 35% of maximal activity, 25°C: about 65% of maximal activity Methylotuvimicrobium alcaliphilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Methylotuvimicrobium alcaliphilum

pH Range

pH Minimum pH Maximum Comment Organism
8 10.5 pH 8: about 50% of maximal activity, pH 10.5: about 70% of maximal activity Methylotuvimicrobium alcaliphilum