Cloned (Comment) | Organism |
---|---|
into the pET22b+ vector for expression in Escherichia coli BL21DE3 cells | Methylophaga thalassica |
into the pET22b+ vector for expression in Escherichia coli BL21DE3 cells | Methylophaga alcalica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Methylophaga alcalica | |
Ca2+ | - |
Methylophaga thalassica | |
Cd2+ | - |
Methylophaga alcalica | |
Cd2+ | - |
Methylophaga thalassica | |
Cu2+ | - |
Methylophaga alcalica | |
Cu2+ | - |
Methylophaga thalassica | |
Mg2+ | - |
Methylophaga alcalica | |
Mg2+ | - |
Methylophaga thalassica | |
Ni2+ | - |
Methylophaga alcalica | |
Ni2+ | - |
Methylophaga thalassica | |
Zn2+ | - |
Methylophaga alcalica | |
Zn2+ | - |
Methylophaga thalassica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
L-2,4-diaminobutanoate | - |
Methylophaga thalassica | |
0.375 | - |
L-2,4-diaminobutanoate | - |
Methylophaga alcalica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19700 | - |
calculated molecular mass | Methylophaga thalassica |
20000 | - |
calculated molecular mass | Methylophaga alcalica |
20000 | - |
determined by gel filtration and SDS-PAGE | Methylophaga thalassica |
20000 | - |
determined by gel filtration and SDS-PAGE | Methylophaga alcalica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-2,4-diaminobutanoate | Methylophaga thalassica | - |
CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
acetyl-CoA + L-2,4-diaminobutanoate | Methylophaga alcalica | - |
CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylophaga alcalica | A9YZY0 | - |
- |
Methylophaga thalassica | A9YZX5 | - |
- |
Purification (Comment) | Organism |
---|---|
using a Ni2+-nitrilotriacetic acid-agarose and a Blue Sepharose 6FF column | Methylophaga thalassica |
using a Ni2+-nitrilotriacetic acid-agarose and a Blue Sepharose 6FF column | Methylophaga alcalica |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris-HCl, pH 8.5, 1 month | Methylophaga thalassica |
4°C, 50 mM Tris-HCl, pH 8.5, 1 month | Methylophaga alcalica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-2,4-diaminobutanoate | - |
Methylophaga thalassica | CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
acetyl-CoA + L-2,4-diaminobutanoate | - |
Methylophaga alcalica | CoA + N4-acetyl-L-2,4-diaminobutanoate | - |
? | |
acetyl-CoA + L-2,4-diaminobutanoate | - |
Methylophaga thalassica | CoA + (2S)-4-acetamido-2-aminobutanoate | - |
? | |
acetyl-CoA + L-2,4-diaminobutanoate | - |
Methylophaga alcalica | CoA + (2S)-4-acetamido-2-aminobutanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Methylophaga thalassica |
homodimer | - |
Methylophaga alcalica |
Synonyms | Comment | Organism |
---|---|---|
DABAAT | - |
Methylophaga alcalica |
diaminobutyrate acetyltransferase | - |
Methylophaga thalassica |
diaminobutyrate acetyltransferase | - |
Methylophaga alcalica |
diaminobutyric acid acetyltransferase | - |
Methylophaga thalassica |
diaminobutyric acid acetyltransferase | - |
Methylophaga alcalica |
EctA | - |
Methylophaga thalassica |
EctA | - |
Methylophaga alcalica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay | Methylophaga thalassica |
30 | - |
activity assay | Methylophaga alcalica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Methylophaga thalassica |
9.5 | - |
- |
Methylophaga alcalica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | - |
Methylophaga thalassica |
7 | 10.5 | - |
Methylophaga alcalica |