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Literature summary for 2.3.1.178 extracted from

  • Mustakhimov, I.I.; Rozova, O.N.; Reshetnikov, A.S.; Khmelenina, V.N.; Murrell, J.C.; Trotsenko, Y.A.
    Characterization of the recombinant diaminobutyric acid acetyltransferase from Methylophaga thalassica and Methylophaga alcalica (2008), FEMS Microbiol. Lett., 283, 91-96.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the pET22b+ vector for expression in Escherichia coli BL21DE3 cells Methylophaga thalassica
into the pET22b+ vector for expression in Escherichia coli BL21DE3 cells Methylophaga alcalica

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Methylophaga alcalica
Ca2+
-
Methylophaga thalassica
Cd2+
-
Methylophaga alcalica
Cd2+
-
Methylophaga thalassica
Cu2+
-
Methylophaga alcalica
Cu2+
-
Methylophaga thalassica
Mg2+
-
Methylophaga alcalica
Mg2+
-
Methylophaga thalassica
Ni2+
-
Methylophaga alcalica
Ni2+
-
Methylophaga thalassica
Zn2+
-
Methylophaga alcalica
Zn2+
-
Methylophaga thalassica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
L-2,4-diaminobutanoate
-
Methylophaga thalassica
0.375
-
L-2,4-diaminobutanoate
-
Methylophaga alcalica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19700
-
calculated molecular mass Methylophaga thalassica
20000
-
calculated molecular mass Methylophaga alcalica
20000
-
determined by gel filtration and SDS-PAGE Methylophaga thalassica
20000
-
determined by gel filtration and SDS-PAGE Methylophaga alcalica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + L-2,4-diaminobutanoate Methylophaga thalassica
-
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
?
acetyl-CoA + L-2,4-diaminobutanoate Methylophaga alcalica
-
CoA + N4-acetyl-L-2,4-diaminobutanoate
-
?

Organism

Organism UniProt Comment Textmining
Methylophaga alcalica A9YZY0
-
-
Methylophaga thalassica A9YZX5
-
-

Purification (Commentary)

Purification (Comment) Organism
using a Ni2+-nitrilotriacetic acid-agarose and a Blue Sepharose 6FF column Methylophaga thalassica
using a Ni2+-nitrilotriacetic acid-agarose and a Blue Sepharose 6FF column Methylophaga alcalica

Storage Stability

Storage Stability Organism
4°C, 50 mM Tris-HCl, pH 8.5, 1 month Methylophaga thalassica
4°C, 50 mM Tris-HCl, pH 8.5, 1 month Methylophaga alcalica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-2,4-diaminobutanoate
-
Methylophaga thalassica CoA + N4-acetyl-L-2,4-diaminobutanoate
-
?
acetyl-CoA + L-2,4-diaminobutanoate
-
Methylophaga alcalica CoA + N4-acetyl-L-2,4-diaminobutanoate
-
?
acetyl-CoA + L-2,4-diaminobutanoate
-
Methylophaga thalassica CoA + (2S)-4-acetamido-2-aminobutanoate
-
?
acetyl-CoA + L-2,4-diaminobutanoate
-
Methylophaga alcalica CoA + (2S)-4-acetamido-2-aminobutanoate
-
?

Subunits

Subunits Comment Organism
homodimer
-
Methylophaga thalassica
homodimer
-
Methylophaga alcalica

Synonyms

Synonyms Comment Organism
DABAAT
-
Methylophaga alcalica
diaminobutyrate acetyltransferase
-
Methylophaga thalassica
diaminobutyrate acetyltransferase
-
Methylophaga alcalica
diaminobutyric acid acetyltransferase
-
Methylophaga thalassica
diaminobutyric acid acetyltransferase
-
Methylophaga alcalica
EctA
-
Methylophaga thalassica
EctA
-
Methylophaga alcalica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
activity assay Methylophaga thalassica
30
-
activity assay Methylophaga alcalica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
Methylophaga thalassica
9.5
-
-
Methylophaga alcalica

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5
-
Methylophaga thalassica
7 10.5
-
Methylophaga alcalica