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Literature summary for 2.3.1.18 extracted from

  • Fried, V.A.
    lac Thiogalactoside transacetylase of Escherichia coli K-12 and ML (1980), J. Bacteriol., 143, 506-509.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.18

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
 acetyl-CoA E. coli K12 Escherichia coli
0.049
-
 acetyl-CoA E. coli ML308 Escherichia coli
1200
-
 isopropyl beta-D-thiogalactoside E. coli K12 Escherichia coli
1500
-
 isopropyl beta-D-thiogalactoside E. coli ML308 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24800
-
 2 * 24800, strain K12 and ML308, SDS-PAGE Escherichia coli
47900
-
 strain K12, high-speed equilibrium centrifugation using meniscus depletion technique Escherichia coli
49900
-
 strain ML308, high-speed equilibrium centrifugation using meniscus depletion technique Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli it is suggested that the enzyme plays an important role in lactose utilization ?
-
 ?
additional information Escherichia coli ML308 it is suggested that the enzyme plays an important role in lactose utilization ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 K12
-
Escherichia coli
-
 ML308
-
Escherichia coli ML308
-
 ML308
-

Purification (Commentary)

Purification (Comment) Organism
K12 strain H30000 Escherichia coli
strain ML308 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + isopropyl beta-D-thiogalactoside
-
 Escherichia coli CoA + isopropyl 6-O-acetyl-beta-D-thiogalactoside
-
 ?
acetyl-CoA + isopropyl beta-D-thiogalactoside
-
 Escherichia coli ML308 CoA + isopropyl 6-O-acetyl-beta-D-thiogalactoside
-
 ?
additional information it is suggested that the enzyme plays an important role in lactose utilization Escherichia coli ?
-
 ?
additional information it is suggested that the enzyme plays an important role in lactose utilization Escherichia coli ML308 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 24800, strain K12 and ML308, SDS-PAGE Escherichia coli