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Literature summary for 2.3.1.180 extracted from

  • Smirnova, N.; Reynolds, K.A.
    Engineered fatty acid biosynthesis in Streptomyces by altered catalytic function of beta-ketoacyl-acyl carrier protein synthase III (2001), J. Bacteriol., 183, 2335-2342.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
subcloning in Escherichia coli strain TG-2, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Streptomyces glaucescens

Protein Variants

Protein Variants Comment Organism
C122A site-directed mutagenesis, the mutant enzyme expressed in Streptomyces glaucescens generates 75% more straight-chain fatty acids than the wild-type enzyme, plasmid-based expression does not affect Escherichia coli strain TG-2, the mutation causes uncoupling of condensation and decarboxylation reactions Streptomyces glaucescens
C122Q site-directed mutagenesis, the mutant enzyme expressed in Streptomyces glaucescens generates 500% more straight-chain fatty acids than the wild-type enzyme, plasmid-based expression does not affect Escherichia coli strain TG-2, the mutation causes uncoupling of condensation and decarboxylation reactions Streptomyces glaucescens
C122S site-directed mutagenesis, the mutant enzyme expressed in Streptomyces glaucescens generates 100% more straight-chain fatty acids than the wild-type enzyme, plasmid-based expression does not affect Escherichia coli strain TG-2, the mutation causes uncoupling of condensation and decarboxylation reactions Streptomyces glaucescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + malonyl-[acyl-carrier protein] Streptomyces glaucescens the enzyme initiates the fatty acid biosynthesis, overview acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces glaucescens
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Streptomyces glaucescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Streptomyces glaucescens acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
acetyl-CoA + malonyl-[acyl-carrier protein] the enzyme initiates the fatty acid biosynthesis, overview Streptomyces glaucescens acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Synonyms

Synonyms Comment Organism
ACO synthase III
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 Streptomyces glaucescens
beta-ketoacyl-acyl carrier protein synthase III
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 Streptomyces glaucescens
KASIII
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 Streptomyces glaucescens