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Literature summary for 2.3.1.180 extracted from
- beta-Ketoacyl-acyl carrier protein synthase III from pea (Pisum sativum L.): properties, inhibition by a novel thiolactomycin analogue and isolation of a cDNA clone encoding the enzyme (2003), Planta, 216, 752-761.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, phylogenetic tree, expression as GST-fusion enzyme in Escherichia coli | Pisum sativum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| thiolactomycin | uncompetitive, IC50 is 0.7 mM | Pisum sativum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.009 | - |
malonyl-[acyl-carrier protein] | pH 7.6, 30°C | Pisum sativum | |
| 0.016 | - |
acetyl-CoA | pH 7.6, 30°C | Pisum sativum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
2 * 42000, about, DNA sequence calculation | Pisum sativum |
| 63000 | 75000 | gel filtration | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + malonyl-[acyl-carrier protein] | Pisum sativum | - |
acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | Q9FSD6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 147fold from leaves by ammonium sulfate fractionation, ion exchange chromatography, hydrophobic interaction chromatography on a butyl resin, and affinity chromatography, recombinant GST-fusion enzyme from Escherichia coli by glutathione affinity chromatography, GST is cleaved off by thrombin | Pisum sativum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetyl-CoA + a malonyl-[acyl-carrier protein] = an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | catalytic residues are Cys123, His323, and Asn353 | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Pisum sativum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Pisum sativum | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| additional information | the enzyme shows also acetyl-CoA-[acyl-carrier-protein] transacylase activity | Pisum sativum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42000, about, DNA sequence calculation | Pisum sativum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACP synthase III | - |
Pisum sativum |
| beta-ketoacyl-acyl carrier protein synthase III | - |
Pisum sativum |
| KAS III | - |
Pisum sativum |
| short-chain condensing enzyme | - |
Pisum sativum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Pisum sativum |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
uncompetitive, IC50 is 0.7 mM | Pisum sativum | thiolactomycin | |
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