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Literature summary for 2.3.1.180 extracted from

  • Mueller, A.P.; Nomura, C.T.
    Mutations to the active site of 3-ketoacyl-ACP synthase III (FabH) increase polyhydroxyalkanoate biosynthesis in transgenic Escherichia coli (2012), J. Biosci. Bioeng., 113, 300-306.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli K-12 MG1655 Escherichia coli

Protein Variants

Protein Variants Comment Organism
F87C binding pocket mutation Escherichia coli
F87C/H244A H244 plays a key role in stabilizing the oxyanion generated in the condensation reaction through hydrogen bonding Escherichia coli
F87I/H244A H244 plays a key role in stabilizing the oxyanion generated in the condensation reaction through hydrogen bonding Escherichia coli
F87S binding pocket mutation Escherichia coli
F87S/N274A N274 plays a key role in stabilizing the oxyanion generated in the condensation reaction through hydrogen bonding Escherichia coli
F87T binding pocket mutation Escherichia coli
F87T/H244A H244 plays a key role in stabilizing the oxyanion generated in the condensation reaction through hydrogen bonding Escherichia coli
H244A H244 plays a key role in stabilizing the oxyanion generated in the condensation reaction through hydrogen bonding, in the mutant stabilization is reduced inhibiting the condensation reaction, leading to an increase in transacylase activity, allowing larger substrates to be subjected to acyl-ACP to acyl-CoA transacylation Escherichia coli
H244A/N274A N274 and H244 play key roles in stabilizing the oxyanion generated in the condensation reaction through hydrogen bonding Escherichia coli
N274A N274 plays a key role in stabilizing the oxyanion generated in the condensation reaction through hydrogen bonding Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-oxoacyl-ACP + acetyl-CoA
-
Escherichia coli acetyl-ACP + 3-ketoacyl-CoA
-
?
acetoacetyl-ACP + acetyl-CoA
-
Escherichia coli acetyl-ACP + acetoacetyl-CoA
-
?
malonyl-ACP + acetyl-CoA
-
Escherichia coli CO2 + CoA + acetoacetyl-ACP
-
?

Synonyms

Synonyms Comment Organism
3-ketoacyl-ACP synthase III
-
Escherichia coli